啶虫脒胁迫对番茄潜叶蛾保护酶及解毒酶活性的诱导效应

贾尊尊; 张旭艳; 叶晓琴; 付开赟; 丁新华; 王小武; 张晓玉; 牙森·沙力; 吐尔逊·阿合买提; 郭文超

doi:10.6048/j.issn.1001-4330.2026.05.004

啶虫脒胁迫对番茄潜叶蛾保护酶及解毒酶活性的诱导效应

Induction effect of acetamiprid stress on the activities of protective enzymes and detoxification enzymes in Tuta absoluta

摘要

摘要:
目的研究啶虫脒亚致死剂量（LC₁₀和LC₂₅）对番茄潜叶蛾保护酶（SOD、POD、CAT)与解毒酶（CarE、GST、MFO）活性的时间动态调控模式，分析啶虫脒亚致死剂量胁迫下番茄潜叶蛾的诱导反应模式。
方法通过浸叶法明确啶虫脒对二龄幼虫的亚致死剂量，分别在处理后1、6、12、24、48、72 h取样，检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST)、多功能氧化酶(MFO)的酶活性变化。
结果低剂量啶虫脒在初期抑制过氧化物酶（POD）活性；过氧化氢酶（CAT）活性随胁迫时间延长逐渐增强；超氧化物歧化酶（SOD）活性表现为抑制-诱导-抑制反应，而高剂量啶虫脒条件下则表现为诱导-抑制-诱导反应；整体抑制羧酸酯酶（CarE）活性，诱导多功能氧化酶（MFO）反应；谷胱甘肽S-转移酶（GST）在低剂量条件下表现为先抑制后诱导的效果，高剂量反应效果不明显。
结论亚致死剂量的啶虫脒在一段时间内可显著影响番茄潜叶蛾的保护酶和解毒酶活性。

Abstract:
Objective To explore the temporal dynamic response patterns of protective enzymes (SOD, POD, CAT) and detoxification enzymes (CarE, GST, MFO) in Tuta absoluta under sublethal concentrations (LC₁₀, LC₂₅) of acetamiprid, and clarify the physiological induction characteristics of the pest under sublethal acetamiprid stress.
Methods The leaf-dipping bioassay was adopted to determine the sublethal concentrations of acetamiprid against the 2nd-instar larvae of Tuta absoluta. Larva samples were collected at 1, 6, 12, 24, 48 and 72 h after chemical treatment, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), carboxylesterase (CarE), glutathione S-transferase (GST) and mixed-function oxidase (MFO) were measured and compared.
Results Low-concentration acetamiprid inhibited POD activity in the early treatment stage; CAT activity increased gradually with the extension of stress time. The change trend of SOD activity under low acetamiprid concentration presented "inhibition-induction-inhibition", while it showed "induction-inhibition-induction" under high concentration. Acetamiprid generally inhibited CarE activity but induced MFO activity. GST activity was first suppressed then induced under low concentration, while no obvious fluctuation was observed under high concentration.
Conclusion Sublethal acetamiprid can significantly alter the activity of protective and detoxification enzymes in Tuta absoluta within a certain period of time.

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