Glycyrrhiza uralensis种子破除休眠方法研究

Study on breaking seed dormancy methods of Glycyrrhiza uralensis seeds

  • 摘要:
    目的 选取4份不同种源甘草(Glycyrrhiza uralensis)种子作为研究材料,旨在探究其种子萌发特性及破除休眠的方法,为甘草的人工栽培提供科学依据。
    方法 测定供试材料形态特征、千粒重、含水量、吸水率、硬实率及休眠率等指标,采用摩擦种皮、浓硫酸处理和高温热水浸泡3种不同处理,寻求最快速度打破甘草种子休眠的方法。
    结果 甘草种子形状呈卵圆形,颜色为黄褐色,外壳较硬。种子的硬实率高达42%~90%,具有不易透水性,未经处理的甘草种子萌发率为6%~41%,不同种源的甘草种子的萌发率差异较为显著(P<0.05);高温处理并不能有效破除甘草种子的休眠,而摩擦处理和浓硫酸处理均能够明显提高种子的萌发率,并且使萌发时间明显提前(P<0.05)。
    结论 浓硫酸处理是一种有效的休眠破除方法,其中浸泡30 min的浓硫酸处理效果最佳,种子的萌发率可达85%~98%。

     

    Abstract:
    Objective Selected four different sources of Glycyrrhiza uralensis seeds as research materials, aiming to explore the germination characteristics of the seeds and methods to break dormancy, in order to provide a scientific basis for the artificial cultivation of licorice.
    Methods Morphological characteristics, thousand seed weight, moisture content, water absorption rate, hard seed rate, dormancy rate, and other indicators of the test materials were determined. Three different methods, namely friction seed coat, concentrated sulfuric acid treatment, and high-temperature hot water soaking, were used to study their effects on seed germination indicators.
    Results The shape of the licorice seeds is oval, with a yellow-brown color and a hard outer shell. The hard seed rate of the seeds is over 90%, and the seeds are impermeable to water. The germination rate of untreated licorice seeds ranges from 6% to 41%, and the germination rate of licorice seeds from different sources differs significantly (P<0.05). High-temperature treatment cannot effectively break the dormancy of licorice seeds, while friction treatment and concentrated sulfuric acid treatment can significantly increase the germination rate of seeds and advance the germination time significantly (P<0.05).
    Conclusion Concentrated sulfuric acid treatment is an effective method to break dormancy, with the best treatment effect achieved with soaking the seeds in concentrated sulfuric acid for 30 minutes, resulting in a germination rate of 85% to 98%.

     

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