Abstract: 【Objective】 To study tle effects of different temperatures on the content of phenylethanoid glycosides, echinacoside and verbascoside in Cistanche deserticola were studied, and the antioxidant capacity of C. deserticola extractum was also studied. 【Method】 UV spectrophotometric method and HPLC were used to determine the content of phenylethanoid glycosides, echinacoside and verbascoside in C. deserticola with different drying temperatures, different extraction temperatures, different concentration temperatures and different water bath steaming temperatures.The antioxidant capacity of C. deserticola extractum was evaluated by DPPH, ABTS, hydroxyl free radical scavenging capacity test and total reducing power test. 【Result】 The content of phenylethanoid glycosides, echinacoside in C. deserticola dried in the shade was higher than that of sundry. Compared to dried in the shade, the content of above three constituents was the hightst when the drying temperature was 60℃. At 80℃, Three constituents was higher than that of other extraction temperatures．Concentration efficiency was obviously increased with the increase of concentration temperature. When the concentration temperature was increased from 35℃ to 60 ℃, the time of concentration of C. deserticola extract was shortened from 7 h to 0.7 h, at this point, Three constituents in concentrated solution was alsohas the highest concentration. When the C. deserticola extract was steamed by water bath, the steaming temperature increased and the time needed for steaming was obviously shortened. When the steaming temperature increased from 40℃ to 85℃, the time of steaming was shortened from 12 h to 1.2 h, and the three constituents in the C. deserticolaextractum was also the highest.In the range of 0.1-0.5 mg/mL, the clearance rate of C. deserticola extractum on DPPH, ABTS, and hydroxyl radicalswas increased with the increase of concentration. The maximum clearance rate on DPPH, ABTS, and hydroxyl radicals reached 92.23%, 83.23%, 91.26%, respectively,and the IC50 value was 0.017 mg/mL, 0.023 mg/mL, 0.248 mg/mL, respectively. The reducing power of C. deserticola extractumwas slightly below vitamin C in the range of 0.1-0.5 mg/mL. 【Conclusion】 Different temperatures has a great influenceon on the content of phenylethanoid glycosides, echinacoside and verbascoside in the process of drying, extraction, concentration and water bath steaming. C. deserticola extractum has strong antioxidant activity.