Abstract: 【Objective】 Study on biological properties of Verticillium and virulence comparison of 16 fungicides. To clarify the pathogen characteristics, and then to provide a basis for disease prevention and control. 【Methods】 This study determined the main biological properties by myphal growth rate of the pathogen and conducted virulence comparison of 16 fungicides. 【Results】 The pathogen was Char medium; growth temperature range of 5-35 ℃, 25-30 ℃, 57 ℃; optimal carbon and nitrogen sources are lactose and potassium nitrate; pH 4.0-11.0, fastest at pH 11.0 and tolerant to partial acidic environment and best under full light at 24 h. The virulence results showed that 16 agents had significantly different inhibitory effects; 40% and 450 g/L were strong, with EC₅₀ values of 0.0891 mg/L and 0.91 mg/L respectively; 30% oxamycin, 30% phenylpropylene azole, 1.8% simamide acetate（EC₅₀：100-155 mg/L）. 【Conclusion】 The optimal temperature range of malticillium is 25-30℃, acid resistance and the best growth under full light; 40% mycelic, 450 g/L fresh amine and 30% oxamycin have a strong or strong antibacterial effect on pathogens, which can be used as the first choice in field disease prevention test.