Abstract:
【Objective】 The purpose of this study is to determine the species and growth characteristics of potato scab pathogen in Aikule Township, Kashgar Prefecture, Xinjiang, for the potato scab disease affected the yield and quality of potato seriously, and caused great losses to potato industry, especially to virus-free seed potato in Xinjiang.
【Method】 The pathogen was isolated and identified by tissue separation method combined with Koch's rule, microscopic morphology and 16 S rDNA. Then different carbon sources and nitrogen sources were selected to test its growth characteristics.
【Result】 Based on its morphological characteristics and 16S rDNA sequence, the pathogen of this disease was identified as
Streptomyces acidiscabies from this area, which produced white-smooth curved spores with melanin and soluble pigment. Eight kinds of the carbon sources and two kinds of nitrogen sources were tested its growth period, such as glucose, methionine(Met) and histidine(His) etc. It could produce reticulate netlike scab after inoculatedon healthy potato tubers by smearing the tuber skin with spores suspension. The optimum growth temperature was 30℃, the pH was 7, and the optimum carbon and nitrogen sources were glucose and methionine separately.
【Conclusion】 The pathogen of the potato scab are isolated and identified from this area, and its growth characteristics were proved in this study, which would be the beneficial research basis for controlling this disease in Xinjiang.