Abstract: 【Objective】 This experiment aims to investigate the effects of concentrate supplementation on body weight gain, blood biochemical indices and reproductive hormones of fattening donkeys, so as to provide reference for improving fattening efficiency of local donkeys in Xinjiang. 【Methods】 Forty healthy female donkeys aged 3-5 years with similar body weight (186.65±10.39) kg were randomly divided into control group and experimental group with 20 donkeys in each group. The control group was fed according to the conventional feeding and management method of fattening donkeys by Jinhuyang Animal Husbandry Co., Ltd., and the experimental group was fed the concentrate supplement 2 kg/d provided in this study under the same feeding and management conditions. The fattening experiment lasted for 90 d, and the weight of fattening donkeys was weighed regularly. 【Results】 The results showed as follows: (1) The total weight gain and daily gain of female donkeys in concentrate and supplement groups were significantly higher than those in control group (P<0.05), which were increased by 94.89% and 100.00%, respectively. (2)The contents of total protein and albumin in plasma of female donkeys were significantly increased by 49.79% and 34.31%, respectively (P<0.05). (3) Supplementation significantly increased plasma triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol were increased by 4.6%, 7.92% and 7.25%, respectively (P<0.05). (4) There was no significant difference in enzyme content between experimental group and control group (P>0.05), but the plasma enzyme level of experimental group was higher than that of control group. 【Conclusion】 Feeding concentrate supplement to fattening donkey can significantly promote the increase of body weight, improve protein metabolism and promote the overall fattening effect of fattening donkey.