Cloning and Bioinformatics Analysis of Salt-tolerance of PsaH Gene from Salicornia europaea

【 Objective 】 To clone a novel PsaH gene from Salicornia europaea, and analyze its biological information for better understanding its role in function of salt-tolerance.【 Method 】Salicornia europaea was used as the plant material to clone the full-length cDNA sequence of SePsaH by RT-PCR. The encoding region and amino acid sequence of PsaH gene, and the structure and function of protein encoded by protein were analyzed by NCBI, MEGA and Expasy and other online Bioinformatics bioinformatics software for SePsaH gene.【 Result 】 Full-length cDNA sequence encoding photosystem I reaction center subunit H was cloned from Salicornia europaea and designated by the name of SePsaH, which was an opening reading frame of 438bp encoding 145 amino acids. The putative protein molecular weight was 15.3kD and its theoretical isoelectric points was 9.84, SePsaH was a hydrophilic protein; Phylogenetic analysis showed that SePsaH gene and Spinacia oleracea were closely related; Through the conservative analysis of the protein, we found that there were 4 conserved domain structures.【 Conclusion 】SePsaH gene was cloned, which has laid the foundation for further study on the gene function and the role in salt-tolerance of Salicornia europaea.