Using SRAP Markers for Rapid Detection of Zaojia(84-24) Watermelon Hybrid Seed Purity

【 Objective 】 To screen out one SRAP marker to detect seed purity,the watermelon hybrids of Zaojia (84-24) and its paternal and female lines were used to study the polymorphorrism of amplifiable spectral bands.【 Method 】Polymorphic primer pairs were selected from 28 SRAP primer pairs for Zaojia (84-24) hybrids and their parents in this experiment. The SRAP primers with good polymorphism, clear bands and stable primers were selected as candidate markers, and the purity of the 100 hybrid seeds was tested and verified.【 Result 】The results showed that 28 pairs of primers had 22 pairs of primers, and polymorphic bands were amplified between F₁ and its parents. The ratio of polymorphism was 78.6%, among which, there was only one pair of complementary type SRAP primer(Me6F/ Em2R)with male display and lack of female parent, The result showed the purity of hybrid seeds was 99.0%, which was basically the same as the field morphological identification (99.1%).【 Conclusion 】SRAP primer (Me6F/Em2R), in combination with Polyacrylamide gel electrophoresis can be used to detect the purity of Zaojia (84-24) hybrid seed rapidly and accurately. There is a great prospect of application in using this method.