Coupling Mechanism between -Resveratrol Accumulation Induced by FeCl₃ Solution and Halliwell-Asada Pathway in Grapevine

【 Objective 】 FeCl₃ solution causes the accumulation of resveratrol, but the mechanism is not clear. Here we aim to study the relationship between the Halliwell-Asada (H-A) pathway and resveratrol accumulation from the perspective of damage protection.【 Method 】After FeCl₃ solution treatment of grape leaves, the degree of cell damage and the level of reactive oxygen species were detected, and the expression of resveratrol and H-A pathway related genes were analyzed.【 Result 】The results showed that the oxidative stress level of leaves treated with FeCl₃ solution were positively correlated with the damage of foliar tissue. A significant resveratrol accumulation was also observed. In addition, the maximum content of resveratrol reached 36.83 μg/g after treatment in a dose-dependant manner. The gene expression levels of resveratrol metabolic enzymes and key enzymes in H-A pathway were increased in FeCl₃ treatment, such as PAL, C4H, 4CL, STS, APX and MDAR except DHAR. Resveratrol contents in H₂O₂ co-treatment were raised, contrary to co-treatments of antioxidant reagents NAC and GSH-EE.【 Conclusion 】FeCl₃ solution decreased ROS scavenging capability of H-A pathway, thus causing oxidative stress and leading to resveratrol accumulation in grapevine samples, which underlines a correlation between resveratrol metabolism and H-A pathway.