Isolation,Identification and Drug Resistance Detection of Campylobacter Jejuni from Cattle in Partial Areas of Xinjiang

【 Objective 】 To study the prevalence of campylobacter jejuni from cattle in partial areas of Xinjiang and its drug resistance.【 Method 】In this experiment, 171 fecal samples were collected from cattle in Urumqi, Changji and Shihezi. After the initial enrichment of Brucella broth, the culture medium was cultured with two improved media, namely, the improved CCD agar base and the Columbia blood plate, and finally identified by polymerase chain reaction (PCR). Finally, the agar diffusion method recommended by the United States Clinical Laboratory Standardization Institute (CLSI) in 2010 was used for drug susceptibility test.【 Result 】A strain of Campylobacter jejuni (No. N22) was isolated from the Urumqi cattle farm and the detection rate was 1.11%(1/90). A strain of Campylobacter jejuni (No.C25) was identified from Changji cattle farmand the detection rate was 3.33%(1/30).There were no strains of Campylobacter jejuni isolated from Shihezi cattle farm. 2 strains of Campylobacter jejuni were highly sensitive to monocyclic beta lactam and aminoglycoside antibiotics,and showing different degrees ofresistenceto quinolones, macrolides and most of the cephalosporins antibiotics.【 Conclusion 】The infection of campylobacter jejuni was found from cattle in Xinjiang, and the infection strains could be used to prevent and cure with monobactam and aminoglycoside antibiotics. The results can provide a scientific basis for the evaluation of the prevalence of campylobacter jejuni and the prevention and control measures in Xinjiang cattle herds.