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    Crop Genetics and Breeding
    Study on Main Agronomic Traitsand Density Tolerance of Different Maize Varieties under Dense Planting Conditions in Arid Areas of Xinjiang
    YANG Jie, HAN Dengxu, WANG Yejian, Abulaiti Abula, LIANG Xiaoling, XI Haojiang, LI Mingdong, WANG Xian, WEN Liwei
    2020, 57(9): 1569-1577.  DOI: 10.6048/j.issn.1001-4330.2020.09.001
    Abstract ( )   PDF (1942KB) ( )  
    【Objective】 On the basis of density tolerance coefficient, combined with the main agronomic traits, such as plant height, spike height and average yield per plant, the density tolerance of the main maize varieties planted in arid and semi-arid regions of Xinjiang were identified, screened and analyzed in the hope of providing theoretical basis and technical support for the innovation of density tolerance germplasm and the breeding of new varieties.【Method】 11 medium and late-maturing maize varieties, such as Xianyu335, Lianchuang 825 and Xinyu108, were selected as test materials in this experiment, which were the latest national, provincial, or about to be examined into national and provincial regional tests with outstanding performances. Split zone design was adopted, with density as the main treatment and variety as the secondary treatment. 2 density levels were designed (82,500 plants /hm2 and 105,000 plants /hm2). The main agronomic traits such as plant height, spike height, yield and density tolerance were analyzed and studied during the growth period.【Result】 The results showed that: with the increase of planting density, the yield of Xianyu 335 was 19,954.23 kg/hm2 under the low density condition of 82,500 plants /hm2, and that of 105,000 plants /hm2 under the high density condition was 20,131.33 kg/ hm2, and the yield reached the maximum potential under the high density condition. Domestic varieties such as Lianchuang 825, Xinyu 108, Jiushenghe 2468 and MC703 had great yield potential, but the overall yield level was lower than those of foreign varieties. The yield of Jiushenghe 2468 was 17,879.6 kg/hm2 under the low density condition of 82,500 plants/hm2, and 18,003.85 kg/hm2 under the high density condition of 105,000 plants/hm2, performing strong tightness.【Conclusion】 Combined with the comprehensive evaluation of the main agronomic characters, such as yield, plant height, spike height, yield per plant and density tolerance coefficient, Xianyu 335, Xianyu 1225 and other foreign varieties have strong density tolerance, and Lianchuang 825, Xinyu 108, Jiushenghe 2468 and other domestic varieties have better density tolerance.
    Transcriptome Analysis of Floral Organ Differentiation Stage of Two Maize Inbred Lines under Drought Stress
    WANG Yejian, YANG Jie, LIANG Xiaoling, Abulati Abra, HAN Denxu, XI Haojiang, LIU Jun, LI Mingdong
    2020, 57(9): 1578-1585.  DOI: 10.6048/j.issn.1001-4330.2020.09.002
    Abstract ( )   PDF (1360KB) ( )  
    【Objective】 In order to analyze the mechanism of maize tassel to drought stress in the differentiation stage of male flower organs of maize, the identification of drought tolerance related genes at the whole genome level through modern molecular biology and high-throughput sequencing technology will provide a new theoretical basis for drought resistance maize varieties.【Method】 High-throughput transcriptome sequencing by Illumina HiSeq 2000 was conducted using the tassels of drought-tolerant inbred line "PHBA6" and drought-sensitive inbred line "Ji 63" under flower organ differentiation stage to drought stress and normal irrigation for 15 days. The obtained Unigenes were compared with the maize reference genome, and the abundance of gene expression among different samples measured according to transcriptome data by RPKM, and the differentially expressed genes between samples screened by the standard of | log2foldchange | ≥1, P<0.05, FDR≤0.001. Functions and pathway of those differentially expressed genes were also investigated using Gene Onto1ogy (GO) database and KEGG pathway database.【Result】 Compared with samples under normal water treatment, 1,394 and 1,300 significantly differentially expressed genes were detected respectively in drought-tolerant inbred “PHBA6” and drought-sensitive inbred line “Ji 63”, of which 831 differentially expressed genes included 822 genes with the same expression patterns and 9 genes with opposite. In addition to these genes, 563 and 469 genotype-specific drought response genes were detected respectively in the two lines. The items of G0 enrichment for these specific drought response genes not exactly the same. Except for the common items of G0 enriched in stimulus response and the cell wall changing, the drought-tolerant inbred "PHBA6" had some items of G0 enriched in the regulation of hormone synthesis, metabolism of organic nitrogen compounds, and protein binding. The KEGG enrichment analysis illustrated that these pathways of specific drought-responsive genes not exactly the same in the two inbred lines. There some differential genes enriched in the biotin metabolism pathway in drought-tolerant inbred “PHBA6”. In addition, three dehydrin genes and three bZIP, MYB and ERF transcription factors screened differentially expressed in drought-tolerant inbred "PHBA6" under drought stress.【Conclusion】 These specific drought-responsive genes the main reason for drought tolerance difference between the two 3 dehydrin genes and three bZIP, MYB and ERF transcription factors which were screened in drought-tolerant inbred "PHBA6" may have important utilization value in the breeding of drought resistance maize varieties.
    The Relationship between Harvest Time and Vigor of Early Maize Hybrid Seed in Xinjiang Seed Production Area
    HAN Dengxu, YANG Jie, WANG Yejian, XI Haojiang, LIANG Xiaoling, LI Mingdong, Abulaiti Abula
    2020, 57(9): 1586-1595.  DOI: 10.6048/j.issn.1001-4330.2020.09.003
    Abstract ( )   PDF (2019KB) ( )  
    【Objective】 To study the relationship between seed harvesting time and seed vigor of early-maturing maize hybrids in Xinjiang seed production base in the hope of providing theoretical basis for seed production enterprises to produce high-quality seeds.【Methods】 Early main maize varieties Xinyu 29, Xinyu 35 and Xinyu 54 in Xinjiang were chosen for hybrid seed production, after pollination every three days, they were harvested, and their grain moisture contents were determined. After the seeds were dried, maize seed vigor was evaluated by standard germination, cold test, field seedling emergence identification and relative conductivity measurement.【Results】 There were differences in water content, standard germination rate, cold test germination rate, field emergence rate and relative electrical conductivity of maize seeds in different harvesting periods. The content of Xinyu 35 was greater than that of Xinyu 29, the conetent of Xinyu 29 was greater than that of Xinyu 54 in terms of physiological dehydration rate. The grain-filling rate of Xinyu 29 was greater than that of Xinyu 54, while that of Xinyu 54 was greater than Xinyu 35's. The seeds of Xinyu 29 were harvested 48-63 d after pollination, the standard germination rate was above 93.3%, cold test germination rate was above 92.0% and the actual seedling emergence rate was above 89.0%. The seeds of Xinyu 35 were harvested 48-63 d after pollination, the standard germination rate was above 93.3%, cold test germination rate was above 90.0% and the actual seedling emergence rate was above 89.0%. The seeds of Xinyu 54 were harvested 48-63 d after pollination, the standard germination rate was above 94.7%, cold test germination rate was above 90.0% and the actual seedling emergence rate was above 89.3%.【Conclusion】 In the early-maturing seed production area of Xinjiang, the days after pollination can be used as a reference index to guide the field harvest of hybrid seeds, so as to obtain hybrid seeds with high vigor.
    Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Cultivation Physiology·Physiology and Biochemistry
    Effects of Different Factors on Early-maturing Upland Cotton Excised Embryos into Seedlings
    Li Chunping, Lai Chengxia, Xu Haijiang, Zhang Jianguo, Mayila Yusuyin, Zhang Dawei, Wei xin, Liu Zhongshan
    2020, 57(9): 1596-1603.  DOI: 10.6048/j.issn.1001-4330.2020.09.004
    Abstract ( )   PDF (1114KB) ( )  
    【Objective】 In vitro embryo culture can effectively shorten the time of crop breeding, especially in the generation-adding technique of cotton breeding, which has important application potential. This project aims to study the effects of different factors on seedling formation of cotton excised embryos, and explore the combination of key factors in the hope of providing theoretical basis for the large-scale cultivation of cotton excised embryo.【Method】 The effects of culture mediums, embryo ages, temperatures and illumination times on cotton embryo seedling formation were studied to analyze embryo seedling rate, seedling time and the development characteristics of root, in order to make clear the suitable and simplified cultivation conditions.【Result】 Among different culture media, MS(Murashige and Skoog)medium had the highest seedling rate, followed by tap water and distilled water. With the increase of embryo age, the seedling rate increased gradually; when the embryo age was 35 d (day), there was no significant difference among the three culture medium. Among different embryo ages, the seedling rate of 35 d was the highest, and the seedling time was the shortest. When the embryo age was 35 d, 30℃ was the most suitable temperature for shortening seedling time and promoting root development. Illumination time 24 h (hour) was beneficial to shorten seedling time, but was not conducive to root development.【Conclusion】 Culture medium, embryo age, temperature and illumination time are the key factors that affect the seedling formation of cotton excised embryo. Advanced embryo age, suitable temperature and illumination time are beneficial to the seedling formation of excised embryo, shortening seedling time and promoting root development. Under the conditions of embryo age 35 d, temperature 30℃ and illumination time 24 h, the effect of hydroponics of excised embryo is good, which can be used as the suitable condition of simplified seedling culture of early-maturing upland cotton excised embryo.
    Effects of Salt Stress on Seed Vigor of Gossypium barbadense L.
    SU Xiujuan, XU Haijiang, DENG Xiaojuan, GONG Lintao
    2020, 57(9): 1604-1614.  DOI: 10.6048/j.issn.1001-4330.2020.09.005
    Abstract ( )   PDF (2593KB) ( )  
    【Objective】 The aim of this study is to reveal the salt tolerance characteristics of different genotypes of Gossypium barbadense L. during germination, and explore the variation rule of its seed vitality index under salt stress in the hope of providing support for the cultivation of salt-tolerant varieties.【Method】 35 sea-island seeds were used as test materials, and salt stress conditions were simulated by NaCl solution (150 mmol/L) in this experiment. Six indicators of germination potential, germination rate, root length, hypocotyl length, fresh weight and dry weight were statistically analyzed.【Result】 The results showed that: (1) Under salt stress, the indexes of Gossypium barbadense L. seed were affected differently, among which hypocotyls were most affected and dry weight was the least affected; (2) Compared with the control samples, the maximum, minimum and average of the five indexes of germination potential, germination rate, root length, hypocotyl length and seedling fresh weight were lower than those of the control, while the maximum, minimum and average values of seedling dry weight were higher than those of the control. 【Conclusion】 Cluster analysis results showed that 35 Gossypium barbadense L.seeds were divided into three categories: the first category belonged to high salt tolerance varieties, the second category belonged to medium salt tolerance varieties, and the third category belonged to low salt tolerance varieties.
    Variance Analysis and Grey Correlation Analysis of Xinsaili Effect on Synthetical Properties of Cotton
    ZHANG Wen, LU Tao, CENG Qintao, CAI Xiaoli, ZHAO Fiqiang, FENG Yang, LIU Quanyi
    2020, 57(9): 1615-1622.  DOI: 10.6048/j.issn.1001-4330.2020.09.006
    Abstract ( )   PDF (1123KB) ( )  
    【Objective】 In order to study the effect of the new defoliant Xinsaili in Kuytun area, the defoliation test was carried out with Z1146 as the test material.【Method】 The defoliation rate, boll opening rate, yield and fiber quality of the new defoliant Xinsaili were studied by variance analysis and grey relational comprehensive evaluation.【Result】 Spraying Saibenlong + ethephon (450 g/hm2+2,250 mL/hm2) and Xinsaili (1,800-2,700 mL/hm2) significantly improved the defoliation rate of cotton, and the ripening effect was better. In terms of cotton yield and cotton fiber quality, spraying defoliation had no significant effect on cotton fiber length, uniformity index and micronaire, but had certain effect on cotton yield and breaking tenacity. The comprehensive traits of all treatments were evaluated by grey correlation method. The order of comprehensive traits of different treatments was T5 > T4 > T3 > T6 > T1 > T2. The comprehensive evaluation value of T5 treatment was the highest, which was 0.861,6.【Conclusion】 T5 treatment (Xinsaili 4: 1,350 +1,350 mL/hm2 sprayed two times) has superior comprehensive performance and is suitable for promotion in Kuytun cotton area.
    Genetic Diversity Analysis of Agronomic Characters in 200 Upland Cotton Germplasm Resources
    WANG Junduo, GONG Zhaolong, LIANG Yajun, AI Xiantao, GUO Jiangping, MO Ming, LI Xueyuan, ZHENG Juyun
    2020, 57(9): 1623-1629.  DOI: 10.6048/j.issn.1001-4330.2020.09.007
    Abstract ( )   PDF (1421KB) ( )  
    【Objective】 To broaden the gene pool of cotton germplasm and screen out the germplasm resources suitable for hybrid parents.【Methods】 the variation of morphological indexes and genetic diversity of 200 cotton germplasms were studied, and the cluster analysis of 200 germplasms carried out.【Results】 The results showed that the variation range of genetic diversity index (H′) of 21 morphological indexes was 0-1.02, and cluster analysis showed that all materials could be divided into 6 categories, of which,among which 30 materials belongs to the first big category with purplish red color stem. Their seeds are mainly of short velvet grayish brown color and can be used as the breeding resources of color cotton; 20 materials of 170177, whose seeds are of short hair green brown color, can be used as the breeding materials of early maturity, phenol free and high temperature tolerance; 50 were in the sixth category, accounting for a large proportion.【Conclusion】 The genetic diversity of 200 germplasm resources is abundant, 50 materials of the sixth class can be used as the parent of breeding materials in Xinjiang at present. It is mainly characterized by tower type of plant type, no stem hair, dark green leaf color, medium leaf size, no leaf base spot, limited branch type, yellow corolla, yellow anther, hairy seed pubescence, gray green seed pubescence, pigment gland of seed kernel and white boll color.
    Effects of Different Fruit Tree Intercropping on Growth Dynamics and Yield of Peanut
    MIAO Haocui, HOU Xianfei, JIA Donghai, GUO Feng, ZHANG Jialei, LI Qiang, LI Limin
    2020, 57(9): 1630-1638.  DOI: 10.6048/j.issn.1001-4330.2020.09.008
    Abstract ( )   PDF (1455KB) ( )  
    【Objective】 To explore the flowering and fruiting, growth and yield of peanut under different intercropping fruit trees.【Method】 Using Huayu 25, Huayu 33, Shanhua7 and Shanhua 9 as test materials, the indicators of peanut flowering and fruiting, dry matter accumulation, and yield characteristics under two fruit trees of jujube and badam were analyzed.【Result】 (1) The main flowering time of peanut under intercropping of different fruit trees was from 10 to 27 days. The total amount of peanut flowering was higher in jujube intercropping than in Badam intercropping.(2) Under the intercropping of jujube, the dry weight accumulation of peanut roots, stems and leaves is vegetative growth performance of peanut under the two intercropping fruit trees at the seedling stage was consistent. the growth period , the ratio of vegetative body to fertility (V/R) and gradually after the maximum seedling stage. (3)Under the same fruit tree intercropping, the main stem height and side branch lengths of different peanut varieties were significantly different. Huayu 22 and Huayu 33 were stronger than Shanhua 7 and Shanhua 9.(4) Fruit number per plant and yield per plant in jujube tree intercropping were higher than those in Badam intercropping.【Conclusion】 The suitable of jujube works Huayu No. 22, and the suitable varieties of Badam are Huayu 22 and Huayu No. 33. Red dates are more suitable for intercropping with peanuts.
    Effects of Different Tree Shapes on Regional Light Environment in Almond-Winter Wheat Intercropping Areas
    ZHANG Wen, ZHOU Hao, LIU Cuirong, ZHOU Yumei, XIE Hui
    2020, 57(9): 1639-1649.  DOI: 10.6048/j.issn.1001-4330.2020.09.009
    Abstract ( )   PDF (2200KB) ( )  
    【Objective】 To study the effects of different tree shapes on regional environment light condition in almond-winter wheat intercropping areas in the hope of providing a theoretical basis for selection and optimization of tree shape with high light efficiency under the fruit-grain intercropping mode in southern Xinjiang.【Methods】 Taking the intercropping mode of almond-winter wheat as the research object, four tree-shaped treatments were set up, including small-canopy shape, open center shape, high-stem shape and stratified shape. During the key growth periods, such as jointing stage, flowering stage and grouting stage, the PAR diurnal variation dynamics and environmental spectral indexes of five intercropping areas, such as far-crown area, near tree canopy area on both east and west sides, under tree canopy area east and west sides were measured and analyzed.【Results】 Under the inter-cropping mode of almond and winter wheat in southern Xinjiang, the diurnal variation of PAR in different intercropping areas showed a single peak curve, the light conditions were obviously deteriorated from flowering stage to jointing stage in all almond trees during treatments, the rate of PAR descent between 2 stages of small-canopy shape, open-center shape, high-stem and trunk stratified form were in turn as 18.93%, 39.06%, 23.24% and 46.00%; The effect of almond tree on the light environment in intercropping area was basically stable at the stage of flowering. At this period, the light PAR intensity in intercropping areas of different tree shapes were divided into three levels, small-canopy shape, high-stem and open-center shape and the stratified shape. Daily PAR strength was within the 400-1 400 mol/(m2·s) range respectively which accounted for about 70%, 40%, and 20% of the day.【Conclusion】 Under the almond - winter wheat intercropping mode, during the jointing stage (8 April) to flowing stage (3 May), the daily mean value of PAR in intercropping areas decreased significantly, it was basically stable from flowering (3 May ) to grouting (25 May) stage without significant change. Based on the PAR daily variation of the intercropping region and the results of the analysis, the PAR daily average intensity and the light quality parameters of the intercropping region showed that the 4 tree shapes can be divided into three categories, the best is small-canopy shape, followed by open-center shape and high-stem shape. The two photoqualitative parameters "red orange/far infrared" and "near red light/far red light" can be used as evaluation indexes for the light environmental conditions in intercropping areas.
    Horticultural Special Local Products·Forestry
    Genetic Diversity Analysis of "Laohan" Muskmelon in Xinjiang Based on Phenotypic Characters
    LI Chao, YANG Ying, ZHENG Heyun, CHEN Wei, LIAO Xinfu, SUN Yuping
    2020, 57(9): 1650-1661.  DOI: 10.6048/j.issn.1001-4330.2020.09.010
    Abstract ( )   PDF (1180KB) ( )  
    【Objective】 To study the phylogenetic relationship and genetic diversity of "Laohan" melon in Xinjiang (soft fleshy texture) by comparing phenotypic traits, in the hope of providing the scientific basis for the evaluation, classification and variety breeding.【Methods】 The frequency distribution, diversity analysis, variation statistics, principal component analysis and cluster detection were conducted, which studied seventeen quality characters and sixteen quantitative characters of fifty-one melon varieties (including forty-five muskmelons, six thin-skinned melons as volunteers) in Xinjiang.【Results】 The mean diversity indexes of quality characters and quantitative characters of the tested melon varieties were 1.06 and 1.91 respectively, indicating that the fruit had richer diversity of quantitative characters. The results of principal component analysis showed that thirty-three phenotypic characters could be summarized into six principal components with a cumulative contribution rate of 74.01%. The fifty-one muskmelon accessions were divided into three groups based on the clustering analysis by igraph software package of R language. Group Ⅰ included thin-skinned melon mainly, Group Ⅱ and Ⅲ were all muskmelons, but the fruits of group Ⅲ were larger than group Ⅱ.【Conclusion】 Fresh fruit quantitative characters of the melon varieties had more rich genetic diversity than quality characters. The genetic diversity of"Laohan"melon variety resources in Xinjiang was relatively rich, which could be divided into two types: large fruit type and small fruit type. In addition, the diversity of quality characters such as flesh color and appearance shape was also rich, which had the full utilization value of new variety breeding and improvement.
    Effects of Picking Leaves and Sepals during Flower Season on the Vascular Bundle Development of Korla Pear and the Relationship with the Tube Detachments
    JIN Min, ZHANG Qian, REN Dandan, CHEN Yan, TAO Shutian, BAO Jianping, WU Cuiyun, ZHANG Rui
    2020, 57(9): 1662-1673.  DOI: 10.6048/j.issn.1001-4330.2020.09.011
    Abstract ( )   PDF (3885KB) ( )  
    【Objective】 To study the effects of different treatments on the development of vascular bundles of fruit stalks, ankles and sacs of Korla Fragrant pears, and the detachment of the stalks during the inflorescence elongation period in the hope of clarifying the differences in the effects of leaves and sepals on the development of vascular bundles.【Method】 The pears with Du pear as the rootstock were used as materials to compare the effects of leaf picking and sepal picking on the shedding rate of different order pear fruits and the number and area of vascular bundles in different parts of the fruit.【Results】 Compared with the control, the leaf shedding rate decreased the first and second order dislocation rates, and the third, fourth, and fifth order dislocation rates increased; the sepal excision treatment reduced the first and the second order dislocation rate, The 3rd position dislocation rate increased, and the influence on the 4th and 5th order was small. The number and area of vascular bundles of fragrant pears differed between different orders and different parts of the fruit. That was, as the inflorescence elongation process progressed, the number and area of vascular bundles of the first and the second orders treated by leaf picking and sepal picking all gradually increased the trend, the number and area of vascular bundles in the 3rd, 4th, and 5th positions of the stem, crotch, and sputum all increased first and then decreased. The results of this study showed that leaf picking promoted the number of vascular bundles in the 4th and 5th orders and the vascular bundle area in the 5th order; The number of vascular bundles in the first to 5th order was improved and the area of the vascular bundle in the first, the second and the third order was enlarged by the sepal picking. (except for that, the area of the vascular bundle in the second order was inhibited).【Conclusion】 Comprehensive comparison showed that leaf picking had a greater inhibitory effect on the vascular bundle development of pear fruits, thereby increasing the rate of fruit tuber detachment. However, the treatment of sepal picking promoted the increase of the number of vascular bundles in the later period and development of fruit stem vascular bundles, thus reducing the rate of fruit tuber shedding.
    Effects of Uniconazole on the Growth of Branches and Leaves and Fruit Quality of Korla Fragrant Pear
    ZHANG Shikui, Abulaike Niyazi, WANG Shaopeng, WANG Yatong, SUN Zhaozhan, FAN Guoquan
    2020, 57(9): 1674-1680.  DOI: 10.6048/j.issn.1001-4330.2020.09.012
    Abstract ( )   PDF (1411KB) ( )  
    【Objective】 The purpose of this study is to explore the effects of Uniconazole treatment with different concentrations on the branch growth and fruit quality of Korla Fragrant Pear in the hope of providing theoretical basis for the fruit cultivation.【Method】 On the 25th day after anthesis, Uniconazole with concentration of 50, 150, 250, 350, 450 mg/L was sprayed on the leaves of the new shoots of Korla Xiangli Pear. The growth indexes such as the length and coarseness of the new shoots were measured regularly, and the fruit quality of each treatment was measured after the fruit matured.【Results】 Uniconazole treatment effectively inhibited the increase of shoot length, internode length, leaf area and chlorophyll value of Korla Fragrant Pear. With the increase of treatment concentrations, the inhibition effect was more obvious, and effectively promoted the increase of shoot diameter. The effect of 150-450 mg/L Uniconazole was obvious. After treatment with Uniconazole, the weight of single fruit, soluble solids, VC content and total soluble sugar of Korla Fragrant Pear were increased to some extents; Meanwhile, with the increase of treatment concentrations, the ratio of stone cells to rough fruits was also increased, and the effect of 450 mg/L Uniconazole was the most significant.【Conclusion】 Spraying 250-350 mg/L of Uniconazole on the leaves during the rapid growth period of the new shoots of Korla Fragrant Pear can effectively inhibit the growth of the new shoots, but has no obvious effect on the fruit quality. It can be applied to the regulation of the growth and development of Korla Fragrant Pear.
    Effects of Different Dwarf Interstocks on Photosynthetic Characteristics of Korla Fragrant Pear
    Abulaike Niyazi, ZHANG Shikui, FAN Guoquan, WANG Shaopeng, WANG Yatong, Du Runqing
    2020, 57(9): 1681-1688.  DOI: 10.6048/j.issn.1001-4330.2020.09.013
    Abstract ( )   PDF (1278KB) ( )  
    【Objective】 To evaluate the effects of Zhongai-1, Zhongai-2, Zhongai-3 and Duli on photosynthetic and fluorescence characteristics of Korla fragrant pear in the hope of providing a theoretical basis for choosing suitable dwarfing interstocks to close planting cultivation of Korla fragrant pear.【Methods】 The photosynthesis and fluorescence parameters of four different dwarfing interstock Korla fragrant pear leaves were measured by using the Li-6400 photosynthesis apparatus and FMS-2 pulse modulation fluorescence instrument, and the differences were analyzed.【Results】 The daily changes of Pn of Korla fragrant pears with different dwarfing interstocks showed a double peak curve, and there was an obvious photosynthetic "noon break" phenomenon, which was caused by non-stomatal limiting factors; The daily mean values of Pn and WUE of Zhongai 1 were higher than those of the other three interstocks; Different dwarfing interstocks had no significant effect on the Gs of Korla fragrant pear; ΦPSⅡ, CNPQ, ETR , Fm, Fv/Fo values of Zhongai 1 were the highest; Different dwarfing interstocks had no significant effect on the Cqp, Fv/Fm values of Korla fragrant pears.【Conclusion】 From the perspective of photosynthesis and chlorophyll fluorescence characteristics, Zhongai 1 as the interstock of Korla fragrant pear is superior to the other two dwarfing interstocks and Duli, which can be considered as an excellent interstock of Korla fragrant pear.
    Relationship between Cell Metabolism Enzyme Activity, Carbohydrate, Endogenous Hormones and Fruit Cracking
    WANG Jianyu, GAO Qiuing, WANG Zhenlei, LIN Minjuan
    2020, 57(9): 1689-1696.  DOI: 10.6048/j.issn.1001-4330.2020.09.014
    Abstract ( )   PDF (1020KB) ( )  
    【Objective】 To understand the relationship between endogenous hormones, carbohydrates and cell metabolic enzyme activity in fruit cracking.【Methods】 Easy to crack varieties 'fucuimi' was taken as the testing material to conduct field experiment in half red stage of dehiscent fruit high crack occurrence periods, which aimed to study dehiscent fruit and normal fruit peel SOD, POD, PPO, cellulase and pectinase, cellulose, hemicellulose, pectin substances and endogenous hormones contents, and fruit of sucrose, starch, soluble total sugar, reducing sugar, glucose, fructose, crestor titration acid and the content of endogenous hormones were determined.【Result】 The activity of POD and SOD in the peel of cracked fruit was significantly higher than that of non-cracked fruit and normal fruit.; The content of hemicellulose, WSP and ISP in the non-cracked pericarp was significantly higher than that in the cracked pericarp, and the content of CSP in the non-cracked pericarp was significantly higher than that in the cracked pericarp; The total soluble sugar, sucrose and starch contents in the pulp of the cracked fruit were significantly higher than those in the non-cracked fruit; The content of GA3 in peel of non-cracked fruit was significantly higher than that peel of cracked fruit; The content of IAA in the pulp of the cracked fruit was significantly higher than that of the non-cracked fruit.【Conclusion】 Changes of hemicellulose content WSP and ISP content as well as POD activity and SOD activity in the peel of the cracked fruit to change the structure of the peel and the properties of the peel, combined with sucrose and soluble total sugar content in the pulp of the change and the endogenous hormone IAA content in the fruit pulp, fruit growth caused by unbalanced and the changes of fruit internal osmotic potential, This causes the fruit to grow incongruously with the skin causing the fruit to crack.
    Effects of Different Sowing Dates in Autumn on the Growth, Development and Quality of Overwintering Spinach in Open Fields
    Adili Abulaiti, JIA Kai, ZHANG Ni, LUO Yanmei, XU Hao, MA Lina, GAO Jie
    2020, 57(9): 1697-1703.  DOI: 10.6048/j.issn.1001-4330.2020.09.015
    Abstract ( )   PDF (1010KB) ( )  
    【Objective】 To study the effects of different sowing dates on the growth, development and quality of overwintering spinach in northern Xinjiang.【Method】 Two spinach varieties , Green Crown 369 and the American Big Leaf , were used as testing materials. According to the change trend of temperature, they were sown on August 15, August 25, September 15, 2018, and the growth index, quality index, yield, bolting time and other related indexes were measured in the spring of the following year.【Results】 Under the condition of open field cultivation, the two spinach varieties could survive the winter safely at three different sowing dates. The oxalic acid content of them decreased with the delay of sowing dates, bolting time was delayed in the next spring, the number of effective leaves was proportional to the increase of effective yield, and the number of effective leaves and effective yield of the two spinach varieties were the lowest after overwintering as early as August 15.【Conclusion】 The effects of different sowing dates on some quality indexes and yields of overwintering spinach in open field are obvious. In order to achieve the purpose of high efficiency and high yield cultivation, according to the local temperature conditions, it is suggested that it is appropriate to sow when the average temperature is reduced to 15-20℃ in northern Xinjiang, generally after August 25.
    Analysis of Fire Severity of Kanas Taiga
    LIU Xiaoju, PAN Cunde
    2020, 57(9): 1704-1712.  DOI: 10.6048/j.issn.1001-4330.2020.09.016
    Abstract ( )   PDF (1644KB) ( )  
    【Objective】 Natural fire disturbance surveys were conducted in Kanas Nature Reserve and the external attribute factors of fire scar in Larix sibirica Ledeb. were used to quantify the fire severity of natural fire disturbance of virgin forest in Kanas Nature Reserve.【Method】 Through field investigation combined with cluster analysis, the fire severity could be quantified into high-, moderate- and low-severity, and each level had its corresponding token factors range, which could be used as the basis for the classification of forest fire intensity in that Nature Reserve.【Result】 In Kanas taiga, plots of high-severity accounted for 37.4%; moderately burned plots accounted for 30.7% and low-severity accounted for 31.8%. The fire history could be attributed to 63 years in between 1880 and 2000. Fire severity levels were no equal before 1990. After 1990 the times of fire decreased and the fire severity was dominated by the moderate and low levels.【Conclusion】 Conclusions were that the natural fires in Kanas Nature Reserve were mainly dominated by the high- and moderate- fire severity. And the fire severity was affected by terrain factors, per-fire stands composition and forest fire management.
    Plant Protection·Soil Fertilizer
    Development and Screening of Seed Coating Agent for Controlling Two Kinds of Root Rot Diseases of Cotton at Seedling Stage
    LIU Mengli, LI Jin, ZHANG Jungao, ZHOU Xiaoyun, DU Pengcheng, WANG LI, ZHAI Menghua, GUO Qingyuani, LEI Bin
    2020, 57(9): 1713-1723.  DOI: 10.6048/j.issn.1001-4330.2020.09.017
    Abstract ( )   PDF (1052KB) ( )  
    【Objective】 To develop a seed coating agent which can effectively control two kinds of root rot diseases of cotton at seedling stage: Rhizoctonia solani K. and Fusarium verticillioides spp.【Method】 The raw drugs of carboxin and fludioxonil were selected as the main components, the growth rate method was used to determine the bacteriostatic effects of different seed coating formulations on two kinds of pathogenic bacteria, indoor germination and pot experiments were used to verify its safety and growth-promoting effect on cotton seedlings, and the comparison of disease control and yield increase was carried out indoors and in the field.【Result】 The principal component analysis showed that the bacteriostatic effect and cotton seedling growth-promoting effect of the seed coating agent with the compound ratio of 7.2 carboxin and 1.0 fludioxonil was the best, and it was better than the commercial seed coating agent 20% tolclofos-methyl ·thiram. Through indoor pot inoculation experiments and field verification, it was found that the compound ratio of 7.2∶1.0 had the best effect on disease control and yield increase. Its control effects on opposite blight and red rot were 87.19% and 88.30%, respectively. In the field, the control effects on the two diseases were 75.79% and 66.3%, respectively, and the yield-increasing effect reached 9.11%.【Conclusion】 The best formulation of seed coating agent of carboxin and fludioxonil was 7.2∶1.0. This kind of coating agent was effective to control root rot diseases of cotton at seedling stage, which had a certain yield-increasing effect.
    Preliminary Study on the Causes of Stiffness and Cracking Boll in Xinjiang Cotton Fields
    LI Guoying, JIAO Ruilian, REN Yuzhong, ZHANG Li, WU Gang, LIU Zheng
    2020, 57(9): 1724-1733.  DOI: 10.6048/j.issn.1001-4330.2020.09.018
    Abstract ( )   PDF (1705KB) ( )  
    【Objective】 Since 2015, a so-called stiffness and cracking boll has occurred in cotton fields of Xinjiang, which has caused a great impact on the yield and quality of cotton. So, experiments were carried out to ascertain the cause.【Methods】 The key factor that caused stiffness and cracking cotton bolls in Xinjiang were revealed by distinguishing the symptoms of previouslyreported boll rot disease. In order to find out whether F. intonsa and A. gossypii were harmful, these symptoms were observed by the method of nylon mesh cover for worm-wearing. In order to find out whether the pathogen was infected, the samples were collected in the cotton fields of northern and southern Xinjiang, and their pathogens were isolated, purified and identified. At the same time, the harm of cotton aphid and the relationship between stiff boll and cracking boll were preliminarily investigated.【Results】 The main cause of rust-colored markings on the boll surface of cotton in fields in Xinjiang was F. intonsa. although the simple harm of cotton aphid did not directly from the symptoms of stiffness and cracking bolls, its harm and the resulting wound and its secretion could obviously induce weak parasitic bacteria such as Alternaria spp. and Cladosporium spp. infection and growth, and lead to the occurrence of dark brown or olive green stiffness and cracking bolls in cotton fields of Xinjiang.【Conclusion】 The main cause of rust-colored markings on the boll surface of cotton in fields in Xinjiang was F. intonsa.
    Cross-resistance and Realized Heritability Analysis of Whitefly Bemisia tabaci MEAM1 cryptic Species to Three Neonicotinoids
    LÜ Huanhuan, ZHOU Chengsong, YANG Liu, LIU Zhenyang, MA Deying
    2020, 57(9): 1734-1745.  DOI: 10.6048/j.issn.1001-4330.2020.09.019
    Abstract ( )   PDF (1447KB) ( )  
    【Objective】 To assess the cross-resistance risk of whitefly Bemisia tabaci (Gennadius) MEAM1 cryptic species to three neonicotinoid insecticides and predict the rate of cross-resistance development in the hope of providing the reference for scientific and rational application of insecticides. MEAM1 [Bemisia tabaci (Gennadius)]【Methods】 The cross-resistance strain was developed by group selection, and the realized heritability(h2) of Bemisia tabaci cross-resistance was estimated by Tabashnik's method.【Results】 Results showed that after being selected by imidacloprid, thiamethoxam and acetamiprid for 9 generations under 50%-70% selection pressure, the cross-resistance ratio of MEAM1 cryptic species to the three neonicotinoids differed from each resistance selected strain. The cross-resistance ratio of imidacloprid selected strain to thiamethoxam and acetamiprid increased by 8.72 -fold and 19.21-fold, respectively, which were low and medium cross-resistance levels and their interactive realized heritability h2 were 0.198,1 and 0.285,4, respectively. The cross resistance ratio of acetamiprid strain to imidacloprid and thiamethoxam increased by 10.08-fold and 9.83-fold, respectively, which were low and medium cross-resistance levels and the interactive realized heritability h2 were 0.242,3 and 0.128,1, respectively. The cross-resistance ratio of thiamethoxam selected strain to imidacloprid and acetamiprid increased by 3.12-fold and 3.11-fold, respectively, which were in the sensitivity decrease stage and the cross realized heritability h2 was 0.142,1 and 0.068,0, respectively.【Conclusion】 The risk of the imidacloprid selected strain developing cross-resistance to thiamethoxam was less than that of acetamiprid. The risk of the acetamiprid selected strain developing cross-resistance to thiamethoxam was less than that of imidacloprid. The risk of the thiamethoxam selected strain developing cross-resistance to acetamiprid was less than that of imidacloprid.
    Effects of Nitrogen Reduction on NH3 Volatilization, Nutrient Uptake and Cotton Yield in Drip-irrigated Cotton Fields
    WANG Fangbin, LIU Kai, SUN Jialin, YIN Xin, HOU Zhenan
    2020, 57(9): 1746-1753.  DOI: 10.6048/j.issn.1001-4330.2020.09.020
    Abstract ( )   PDF (1478KB) ( )  
    【Objective】 A field experiment was conducted to evaluate the nitrogen fertilizer reduction on NH3 volatilization, nutrient use and cotton yield in drip-irrigated cotton field.【Methods】 The experiment consisted of 5 fertilization treatments: control (no N fertilization, CK), farmer conventional fertilization (300 kg/hm2, T300), conventional fertilization N reduced by 20% (240 kg/hm2, T240), acid liquid fertilizer N reduced by 20% (240 kg/hm2, L240), acid liquid fertilizer N reduced by 35% (200 kg/hm2, L200).【Result】 The volatilization loss of soil NH3 in nitrogen reduction treatment (T240, L240, L200) was 31.1%, 73.4% and 78.8% lower than that in T300 treatment, respectively. At the same N application level, NH3 volatilization accumulation in L240 treatment was 61.4% lower than that in T240 treatment. The nitrogen uptake of T240 and L240 treatments was significantly better than that of T300 treatment, which increased by 9.1% and 12.6%, respectively, compared with T300 treatment. The phosphorus uptake of cotton treated with L240 was the highest in each treatment, which was 11.7%-17.7% higher than those of other treatments. The yields of cotton in T240 and L240 treatments were significantly higher than that in the T300 treatment, which increased by 9.6% and 12.6%, respectively. Compared with T300 treatment, each nitrogen reduction treatment improved the nitrogen fertilizer utilization rate of cotton, in which the apparent nitrogen fertilizer utilization rate increased by 20.1% to 24.9%.【Conclusion】 Acid liquid fertilizer N reduced by 20% significantly reduced NH3 volatilization loss, promoted nitrogen and phosphorus uptake in cotton and increased cotton yield and fertilizer use efficiency.
    Study on the Effect of Washing and Desalting of Soil in Saline-alkali Land of Xinjiang by the Breaking of the Barrier by the Deep Pine Ridge
    Dilinur Abula, HUANG Jian, QI Tong, FENG Yaozu, WANG Zhiguo
    2020, 57(9): 1754-1761.  DOI: 10.6048/j.issn.1001-4330.2020.09.021
    Abstract ( )   PDF (1960KB) ( )  
    【Objective】 Through the application of the technology and equipment of silt ridge deep breaking barrier in different types of soil, the change of soil salinity at different points before and after irrigation was studied, and the leaching and desalination effect of silt ridge deep breaking barrier technology on saline-alkali land was analyzed.【Methods】 Using the powder ridge deep-breaking equipment, the deep-breaking depth reached 110 cm and the spacing of deep-breaking reached 200 cm in the moderately or above saline-alkali land. Samples were taken before and after winter irrigation. The sampling points were horizontal spacing of 0, 30 and 90 cm, and the sampling depth was 0-30, 30-60 and 60-90 cm, respectively.【Results】 After the barrier was broken by the deep loosening of the bed, the soil desalination rate before and after winter irrigation was: the horizontal direction of the sand soil was 0 cm=78% > 60 cm = 34% > 90 cm = 31%, and the vertical direction was 60-90 cm = 69%> 0-30 cm = 43% > 30-60 cm= 32%. The horizontal direction of loam was 60 cm=22% > 0 cm = 20% > 90 cm = 7%, and the vertical direction was 0-30 cm = 30% >30-60 cm=12% > 60-90 cm = 6%. The horizontal direction of clay was 0 cm = 33% > 60 cm = 29% > 90 cm = 21%, and the vertical direction was 0-30 cm = 40% > 60-90 cm = 27% > 30-60 cm = 17%.【Conclusion】 The highest desalination efficiency was 48%, 28% for clay and 18% for loam. The desalination rate was 44%, 28% at 30 cm and 20% at 90 cm in the barrier area with deep bed loosening. At different depths, the surface desalination rate of 0-30 cm was 38%, that of 30-60 cm was 20%, and that of 60-90 cm was 34%. The effect of washing and desalting of soil can be effectively improved by loosening and breaking the barrier in the bed.