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20 July 2023, Volume 60 Issue 7 Previous Issue    Next Issue
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Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer
Identification and Appraisal of Soybean Germplasm Resources
Horticultural Special Local Products·Storage and Preservation Processing
Facility Agriculture·Plant Protection·Forestry
Microbes·Animal Husbandry Veterinarian


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Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer

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Effects of different delinting modes on seed vigor and seedling characteristics of cotton under PEG stress Mierzhati Mutalifu, SHI Xiunan, BO Junbing, Zubaidai Abudukerimu, Wulejialehasi Azhati, SHI Shubing 2023, 60(7): 1561-1568.  DOI: 10.6048/j.issn.1001-4330.2023.07.001 Abstract ( )   HTML ( )   PDF (718KB) ( )   【Objective】 To study the effects of different delinting modes on cotton seed vigor and seedling characteristics under PEG stress in the hope of providing theoretical support for producing cotton seeds with high vigor.【Methods】 Xinluzao 64 was used as the experimental material and three delinting treatments including T1 (sulfuric acid), T2 (laser) and T3 (laser) were set.Then standard germination test was carried out under four modes CK (0%), P5 (5%), P15 (15%) and P25 (25%) of PEG-8000 concentration.【Results】 With the increase of PEG-8000 concentration, the germination rate, germination potential, germination index, vigor index, dry weight of vegetative organs, plant length, contents of soluble sugar and peroxidase decreased at seedling stage.While the contents of malondialdehyde and proline significantly increased.Simultaneously, the average of stem diameter, volume and average diameter of root increased first and then decreased at seedling stage.Significant effects were found on cotton seed germination rate and germination potential under different delinting methods.Among them, germination rate, germination potential, root volume, average root diameter, the contents of proline and peroxidase showed T1 > T2 > T3 under three delinting methods.The germination index, vigor index, average stem diameter, root length and root surface area were performed as T1 > T3 > T2.The contents of soluble sugar and malondialdehyde indicated T3 > T2 > T1 among three delinting modes.【Conclusion】 Compared with delinting method of sulfuric acid depilation, the germination rate and germination potential of cotton seeds are reduced using laser depilation method, the seed vitality and drought resistance is improved.

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Responses of seed cotton yield and fiber quality of different sea island cotton varieties to nitrogen fertilizer DUAN Songjiang, PENG Zengying, SHEN Yingying, Mulidier Baibolati, WU Yifan, CUI jianping, ZHANG Jusong 2023, 60(7): 1569-1579.  DOI: 10.6048/j.issn.1001-4330.2023.07.002 Abstract ( )   HTML ( )   PDF (955KB) ( )   【Objective】 To study the differences in agronomic traits, seed cotton yield and fiber quality of different island cotton varieties under different nitrogen fertilizer levels in the hope of providing a theoretical basis for the future selection of high-yield and high-quality island cotton varieties and reasonable nitrogen application rates.【Methods】 In the experiment, 12 sea island cotton varieties bred in the past two decades were used as test materials, and three nitrogen application treatments were set N0 (0 kg/hm2), N240 (240 kg/hm2), N480 (480 kg/hm2), to study nitrogen application rates effects on the agronomic characteristics, fiber quality, yield composition and seed cotton yield of different island cotton varieties.【Results】 The results of the study showed that nitrogen application could significantly increase the number of leaves, fruit branches and buds and bolls of island cotton, and the cotton yield by the nitrogen application treatment was higher than that of the non-nitrogen treatment; Among the cultivars, Xinhai 28 and Miancheng 10 performed better in comprehensive agronomic traits, and could maintain a relatively low dropout rate of bolls under different nitrogen fertilizer levels, thus ensuring the number of bolls.Nitrogen fertilizer had a significant effect on the number of bolls per plant and the yield of seed cotton, which was N240>N480>N0, and had no significant effect on other yield traits; among varieties, the number of bolls per plant, boll weight per plant and seed cotton yield were all significant.The difference between the number of bolls per plant and the weight of bolls per plant under different nitrogen levels for Miancheng 10 and Xinhai 28 resulted in higher seed cotton yield.In terms of fiber quality, nitrogen fertilizer only had a significant effect on the average length of the upper half, showing a downward trend with the increase of nitrogen application rate, and had no significant effect on other indicators.However, there were extremely significant differences between varieties, indicating that the fiber quality was mainly affected by genetic characteristics, the fiber length, uniformity, and breaking strength of the upper half of Xinhai 28 and Xinhai 45 performed well under different nitrogen fertilizer levels, and the micronaire value reached the A-level standard.【Conclusion】 In summary, Miancheng 10 and Xinhai 28 have excellent agronomic traits under different nitrogen fertilizer levels, and their yield traits are relatively coordinated.Therefore, higher seed cotton yield and fiber quality can be achieved under different nitrogen fertilizer levels.Regression analysis speculates that when the nitrogen application rate is 332.2 kg/hm2, the seed cotton yield is the highest, which is 5025.1 kg/hm2.

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Features of aged cotton stalk charcoal and its effect on ammonia volatilization from sand soil LIN Ling, ZHU Yujie, FENG Lei, TANG Guangmu, ZHANG Yunshu, XU Wanli 2023, 60(7): 1580-1588.  DOI: 10.6048/j.issn.1001-4330.2023.07.003 Abstract ( )   HTML ( )   PDF (1107KB) ( )   【Objective】 To study on the characteristics of biochar from cotton straw aged with dry-wet alternation, freeze-thaw cycle, citric acid, and explore the effects of aging cotton stalk charcoal on ammonia volatilization from sand soil with a view to providing theoretical basis for the utilization of cotton stalk charcoal and its long-term effects on soil improvement in arid areas.【Methods】 Cotton straw biochar was taken as the test material, three aging methods of dry and wet alternation, freezing and thaw cycle and citric acid were set to analyze the surface morphology of cotton straw carbon and analyze the surface morphology, specific surface area and pore size, pH value, electrical conductivity and other physical and chemical properties of cotton stalk charcoal before and after aging.Meanwhile, the effects of aged cotton stalk charcoal on ammonia volatilization rate and ammonia volatilization accumulation in soil were studied by indoor static soil culture experiment.【Results】 (1) Compared with fresh cotton stalk charcoal, the pH value, electrical conductivity, total phosphorus and total potassium contents of aged cotton stalk charcoal all decreased and its degree of citric acid was greater than those of dry-wet alternation and freeze-thaw cycle.The specific surface area, total pore volume and mesoporous volume of citric acid of aged cotton stalk charcoal increased by 1.88,1.37 and 1.36 folds, respectively and the average pore size decreased by 0.19 times (P> 0.05).The specific surface area of dry-wet and freeze-thaw aged cotton stalk charcoal decreased significantly, but the average pore size increased significantly.(2) In the whole culture process, the cumulative amount of ammonia volatilization was reduced by 7.4%~34.7% compared with the treatment of fresh cotton stalk charcoal.【Conclusion】 Three aging modes of freeze-thaw cycle, alternation of wetting and drying, and citric acid change the characteristics of cotton stalk charcoal, inhibit ammonia volatilization in aeolic soil, which is beneficial to soil nitrogen fixation.The citric acid aged cotton stalk charcoal has the best inhibition effect on ammonia volatilization in soil.

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Effect of hydroponic culture on amino acid composition of whole plant barley and wheat seedlings and comparative analysis YANG Jinyu, WANG Xihe, SUN Jiusheng 2023, 60(7): 1589-1595.  DOI: 10.6048/j.issn.1001-4330.2023.07.004 Abstract ( )   HTML ( )   PDF (675KB) ( )   【Objective】 To clarify the effect of hydroponic culture on the amino acid composition of whole plant barley (Hordeum vulgare L.) and wheat (Triticum aestivum) seedlings, and compare the difference of their nutritional value, so as to provide a basis for the development and utilization of hydroponic green fodder and crop screening.【Methods】 Taking common barley (Ganpi 8) and wheat (Xindong 18) varieties in Xinjiang as the research object, after 3 days of germination and 5 days of hydroponics treatment in a controlled environment, the amino acid content of the stem and root parts of the whole plant of hydroponics barley and wheat were determined respectively. At the same time, the amino acid composition and nutritional value of barley and wheat seeds without germination and hydroponics were analyzed as the control.【Results】 The results showed that compared with the seed, after 5 days of hydroponic culture, the total amino acid content of barley and wheat in the stem and leaf increased by 129% and 102%. The content of other 16 amino acids increased significantly (P<0.05), otherwise the glutamic acid content decreased. The content of lysine increased 3-4 times, and the contents of delicious, sweet, aromatic and effective amino acids increased also; The contents of essential amino acids, total amino acids and special functional amino acids in wheat roots decreased slightly, the content of nonessential amino acids in barley roots decreased by 10%, and the contents of sweet and effective amino acids increased slightly. Compared with the two crops after hydroponics, the contents of 12 kinds of amino acids in the stems and leaves of wheat were the highest(P<0.05), such as lysine, aspartic acid and alanine. The contents of flavor amino acids and effective amino acids were significantly higher than those of barley; The contents of leucine, proline and other five amino acids in barley stem and leaf were the highest (P<0.05). 【Conclusion】 To sum up, after 5 days of hydroponic cultivation, the seeds and seedlings have enriched the amino acid nutrition of the stems and leaves, and the amino acid nutrition value of hydroponic wheat is higher than that of barley.

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Poduction performance screening and nutritional value evaluation of corn varieties suitable for silage WANG Ting, ZHANG Li, ZHANG Fanfan, HUANG Rongzheng, LI Xiao, ZHANG Yulin, CHEN Yongcheng, ZHAO Jiantao, MA Chunhui 2023, 60(7): 1596-1605.  DOI: 10.6048/j.issn.1001-4330.2023.07.005 Abstract ( )   HTML ( )   PDF (808KB) ( )   【Objective】 The purpose of this study is to screen out high-quality silage corn varieties suitable for planting in Xinjiang.【Methods】 17 silage corn varieties (silage-specific, grain-feeding, ordinary) were selected as research objects to carry out variety comparison tests, and analyze the differences in yield, agronomic characters and nutritional quality of the tested varieties.【Results】 The results showed that the growth days of Xinsiyu 13 was the shortest (116 d) and Guisilage 1 was the longest(143 d).The hay yield of Enxiai 298 was the highest (70.33 t/hm2) and Xinyu 15 was the lowest (25.69 t/hm2).The grain yield of Xinwo 1 was the highest (10.75 t/hm2) and Tunyu 168 was the lowest (5.95 t/hm2).Among the agronomic characters, the plant height of Aoyu 5102 was the highest (359 cm) and Xinyu 15 was the lowest (241.67 cm).The ear height of Guisilage 1 was the highest (224.67 cm) and Heyu 187 was the lowest (97.67 cm).The lodging rate of Xinsiyu 13 was the highest (36.98%), Xianyu 335, Zhengdan 958, Xianyu 1321, Xinyin KWS2564, Xinyu 15, Jingke 968 and Xiannuoyu 335 did not lodge.The fold rate of Guisilage 1 was the highest (9.73%), Xinsiyu 13, Xinyin KWS2564, Xinyu 15, Xianyu 1321, Zhengdan 958, Tieyan 53 and Tunyu 168 did not fold.In nutrition, Xinwo 1 was the highest (38.83%) and Heyu 187 was the lowest (24.16%).The Starch content of Xianyu 335 was the highest (32.77%) and Heyu 187 was the lowest (23.75%).The crude protein (CP) content of Jingke 968 was the highest (9.00%) and Xinyu 15 was the lowest (7.47%).The Water-soluble carbohydrate (WSC) content of Xiannuoyu 335 has the highest (41.28%)and Aoyu 5102 has the lowest(13.24%).The Neutral washing fiber (NDF) of Zhengdan 958 was the lowest (43.93%) and Xinsiyu 11 was the highest (55.97%).The acid washing fiber (ADF) of Jingke 968 was the lowest (24.96%) and Aoyu 5102 was the highest (40.56%).The comprehensive value rank was, Xianyu 335>Xianyu 1321>Enxiai 298>Jingke 968>Xinsiyu 13>Xiannuoyu 335>Xinwo 1>Zhengdan 958>Xinsiyu 12>Xinsiyu 11>Tieyan 53>Xinyin KWS2564>Guisilage 1>Tunyu 168>Heyu 187>Xinyu 15>Aoyu 5102.【Conclusion】 Based on the comprehensive analysis of yield and quality, Xianyu 335, Xianyu 1321 and Enxiai 298 were suitable to be popularized and planted as silage corn varieties in Xinjiang.

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QTL mapping and genomic selection of maize leaf width CHEN Zhanhui, SUN Qiang, REN Jiaojiao, HUANG Bowen, XU Jiabo, YANG Jie, WU Penghao 2023, 60(7): 1606-1613.  DOI: 10.6048/j.issn.1001-4330.2023.07.006 Abstract ( )   HTML ( )   PDF (853KB) ( )   【Objective】 To carry out ggenetic analysis of the leaf width of maize and detection of major QTL controlling leaf in the hope of providing an important theoretical basis for breeding maize with ideal plant type.【Methods】 In this experiment, the F2∶3 family was constructed from the maize inbred lines B73 and Zheng 58 which were genotyped by liquid phase 48k probe capture technology and phenotyped in multi-environment trails for QTL mapping and genomic selection.【Results】 The results showed that the leaf width displayed significant differences in genotype, environment, and genotype and environment interaction.The broad-sense heritability was 0.39.A total of 12 QTL distributed on chromosomes 1, 3, 4, 5, 8 and 10 were identified for leaf width.The phenotypic variation explained of each QTL ranged from 3.75% to 16.17%.Two loci, located on bin1.06 and bin5.01, were stable QTL detected in multiple environments.The QTL located on bin 5.01 was a major QTL, which could be used for fine mapping.When the number of SNPs reached 300 and the training population size reached 50%.【Conclusion】 Leaf width is controlled by major polygenes, and genome selection can accelerate the efficiency of leaf wide traits.

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Research progress on sex pheromone identification and application of Asian corn borer LAN Chenyihang, YAO Yubo, ZHOU Junxiang, FU Kaiyun, DING Xinhua, YIN Xiaohui, LIU Wen, WANG Na, GUO Wenchao, DENF Jianyu 2023, 60(7): 1614-1622.  DOI: 10.6048/j.issn.1001-4330.2023.07.007 Abstract ( )   HTML ( )   PDF (1026KB) ( )   【Objective】 Reviewing the domestic and foreign research progress on sex pheromone identification, comprehensive prevention and control and application of Asian corn borer in recent years, in order to provide an important reference for the monitoring, early warning and green prevention and control of Asian corn borer in my country.【Methods】 Statistical methods were used to collect literature data, and the progress of domestic and foreign literature on the research and application of sex pheromone control technology of Asian corn borer was analyzed. 【Results】 The Asian corn borer is distributed in China, South Korea, Japan, the Philippines, etc. Females release sex pheromones to attract males and then mate;The main sex pheromone components of the Asian corn borer were identified at home and abroad as cis/trans-12 tetradecanol acetate (Z/E-12-14:Ac) and n-tetradecyl acetate (14:Ac). The effects of each component were different, and there were geographical population differences in the proportion of components; At present, the sex pheromone products of the Asian corn borer have been widely used in monitoring, mass trapping and mating interference, and the integrated technology combined with other control technologies also has significant control effects; There are many factors affecting the trapping efficiency of sex pheromone lures during the application process, including sex pheromone dosage, trap type, placement height, color and environment, etc. 【Conclusion】 The Asian corn borer is distributed in the corn planting areas of many countries. There are geographical population differences in the proportion of sex pheromone of the Asian corn borer. The best component system of sex pheromone, combined with various factors affecting the trapping efficiency of sex pheromones, has achieved good results in field prevention and control of the Asian corn borer. China has assembled and integrated a set of green control technology based on sex pheromone to prevent and control the Asian corn borer.

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Effect of NaCl stress on seed germination of Panicum miliaceum L. XIAO Jing, LIU Ning, XU Minghai, ZHANG Jinbo, MA Yanming, WANG Li, XU Lin 2023, 60(7): 1623-1629.  DOI: 10.6048/j.issn.1001-4330.2023.07.008 Abstract ( )   HTML ( )   PDF (882KB) ( )   【Objective】 To study the effect of NaCl stress on Seed Germination of chime by analyzing and comparing the salt tolerance of Inner Mongolia chyme 186 and Xinjiang chyme MZ04.【Methods】 In this study, the materials were treated by seed germination test, and the germination potential, germination rate, radicle root length, radicle stem length, germination index and vigor index were calculated.In the process, there were significant differences in germination power, germination rate, germination index, vigor index, radicle and radicle length among the tested materials.【Results】 With the increase of NaCl concentration, each index decreased at 200 and 250 mmol / L.The germination rate, germination potential, germination index and vigor index of Xinjiang chyme MZ04 under mmol / L treatment were significantly higher than those of Inner Mongolia chyme 186 in mainland China, and the radicle and radicle length of Xinjiang chyme MZ04 under different concentrations of NaCl treatment were higher than those of Inner Mongolia chyme 186 in mainland China.Through statistical regression analysis, it was found that during the process of NaCl stress, the concentration of NaCl was negatively correlated, and the concentration of salt tolerance semi inhibition of Xinjiang chyme MZ04 was higher than that of Inner Mongolia chyme 186.The germination rate of Inner Mongolia chyme 186 and Xinjiang chyme MZ04 was 107.39 mmol / L, 4.18 mmol / L higher than that of Inner Mongolia chyme 186, the activity index was 45.81 mmol / L, 5.2 mmol / L higher than that of Inner Mongolia chyme 186.【Conclusion】 In the process of salt stress, the related indexes of germination are mainly determined by salt concentration and salt tolerance of varieties, and the salt tolerance of Xinjiang chyme MZ04 seed in germination period is higher than that of chyme 186.

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Drought resistance identification and physiological changes of different millet varieties during germination BAI Ling, FENG Guojun, HU Xiangwei, ZHAO Yun, SHI Shubing 2023, 60(7): 1630-1640.  DOI: 10.6048/j.issn.1001-4330.2023.07.009 Abstract ( )   HTML ( )   PDF (1280KB) ( )   【Objective】 To explore the impact of drought on millet germination, screen out drought resistance related indicators during millet germination, and further clarify the response mechanism of antioxidant enzymes in different millet varieties to drought stress during germination.the research results can provide a reference for selecting and cultivating millet varieties suitable for planting in Xinjiang.【Methods】 Using 20% polyethylene glycol (PEG-6000) to simulate drought stress during germination, the drought resistance of 15 millet varieties was evaluated, and the changes in the activities of SOD, POD, CAT, and GR of 15 varieties under drought stress were measured to study their internal situation. 【Results】 (1) Under drought stress, the germination potential, germination rate, radicle length, plumule length, fresh weight, and dry weight of the tested millet varieties were inhibited to varying degrees, with a relatively small impact on the germination rate, but the root buds of several varieties such as Mengfenggu 7 had a promoting effect.(2) A comprehensive evaluation of drought resistance was conducted using the membership function method. The drought resistance of 15 millet varieties was graded, including 5 strong drought resistant varieties, 4 moderate drought resistant varieties, 5 drought sensitive varieties, and 1 drought extremely sensitive type. (3) Under drought stress, the activities of antioxidant enzymes SOD, POD, CAT, and GR in millet seedlings of different varieties increased, and the drought tolerant varieties themselves had relatively high antioxidant enzyme activities and could maintain high levels under drought stress. 【Conclusion】 Overall, Mengfenggu 7 has the best drought resistancehe.

Identification and Appraisal of Soybean Germplasm Resources

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Gray correlation analysis of reast soybean yield correlation traits SHI Xiaolei, DING Sunlei, CONG Hua, ZHANG Jinbo, QU Kejia, WANG Xingzhou, HAN dai, YAN Yongliang 2023, 60(7): 1641-1652.  DOI: 10.6048/j.issn.1001-4330.2023.07.010 Abstract ( )   HTML ( )   PDF (844KB) ( )   【Objective】 In order to select the high-yield summer sowing soybean varieties suitable for planting promotion in southern Xinjiang, the agronomic traits closely related to the summer sowing soybean yield were excavated.【Methods】 In 2021, the field comparison test of different replanted soybean varieties was conducted in Zepu County, Kashgar region, and the gray correlation analysis method was used to study the main agronomic traits and yield relationship of 23 summer soybean varieties. 【Results】 The highest yield of Jiyu 354 was 3,271.05 kg/hm2, followed by Jiyu 260 at 3,154.35 kg/hm2 and Jiyu 554 at 3,066.75 kg/hm2. The correlation between soybean yield and major agronomic traits was ranked from large to small as:plant height (0.731)> 100 grain weight (0.714)> bottom pod height (0.699)> pod number (0.678)> Single seed number (0.669)> individual pod number (0.646)> stalk diameter (0.636)> individual seed weight (0.633)> planting density (0.625)> reproductive period (0.614)> main stem branch (0.611). 【Conclusion】 The summer sowing soybean varieties suitable for planting in southern Xinjiang are Jiyu 354、Jiyu 260 and Jiyu 554, the agronomic traits closely related to the yield of summer sowing soybean in Xinjiang are mainly plant height and hundred grain weight.

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Comparative study on the agronomic traits and yields of different soybean varieties YAN Yongliang, ZHANG Heng, QU Kejia, SHI Xiaolei, WANG Xingzhou, ZHANG Jinbo, CONG Hua 2023, 60(7): 1653-1662.  DOI: 10.6048/j.issn.1001-4330.2023.07.011 Abstract ( )   HTML ( )   PDF (945KB) ( )   【Objective】 To identify the adaptability and high yield of introduced soybean varieties (lines) in southern Xinjiang in the hope of providing a scientific basis for the replacement and popularization of wheat stubble soybean varieties in southern Xinjiang.【Methods】 The agronomic traits and yields of 27 new soybean varieties (lines) participating in the experiment were measured, and the methods of variance analysis, correlation analysis, principal component analysis and cluster analysis were used for comprehensive identification and evaluation.【Results】 The variety with the highest yield was SC15-11, with a yield of 266.995 kg/667m2, an increase of 47.872% compared with the control.Yield had a very significant positive correlation with the number of seeds per plant (P<0.01), and a very significant positive correlation with the number of effective pods per plant (P<0.01).When the Euclidean distance was 5.00, all materials could be divided into 3 groups, namely high-yield, middle-yield and low-yield varieties.【Conclusion】 According to the test results, SC15-11, JO14 and Jidou 22 have outstanding comprehensive traits and can be planted for testing in southern Xinjiang.

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Analysis of the main characters of soybean varieties in Xinjiang and their correlation with yield ZHAO Lianjia, LI Gan, XU Lin, YAN Guorong, LIU Ning, WANG Fan, DENG Chaohong, Abudukeyoumu Abudurezike, WANG Cong, WANG Wei 2023, 60(7): 1663-1670.  DOI: 10.6048/j.issn.1001-4330.2023.07.012 Abstract ( )   HTML ( )   PDF (794KB) ( )   【Objective】 In order to rationally evaluate the performance of different soybean varieties in Xinjiang.【Methods】 27 domestic soybean germplasms were selected as the experimental materials. Meanwhile, the coefficient of variation, correlation analysis, principal component analysis, path analysis and cluster analysis were conducted through eleven agronomic traits examination. 【Results】 The results showed that the main soybean quantitative traits have rich variations with the variation coefficient of 8.3% -78.8%. The variation coefficient of branch number is the largest and the variation coefficient of 100-seed weight is the smallest. The yield is has a positive or extremely positive correlation with branch number, pod number per plant, grain number per plant and grain weight per plant. However, the yield has a negative correlation with Height of lowest pod and has an extremely negative correlation with grain number per plant and grain weight per plant. Through principal component analysis, three components were extracted and the cumulative contribution rate reached 75.98%. It was obvious that the main traits affecting yield include grain number per plant、grain weight per plant and 100-seed weight and plant type factor. Stepwise regression analysis was applied to establish the linear regression equation Y=-1,989.985+39.429X8+150.432X10+23.137X9 between yield and grain number per plant, 100-seed weight and grain weight per plant. The determination coefficient was R2=0.887. Based on path analysis, grain number per plant (0.863) and grain weight per plant (0.739) are of more importance than 100-seed weight (0.155) in determining soybean yield. Cluster analysis was adopted. Systematic cluster analysis classified the tested materials into seven divisions at Euclidean distance 15 level. The division I, III and VI have a plant height of about 83 cm and a growth period of about 117 d. They have few branches and high yield. They are of medium-maturing type and are suitable for planting in Yili, Xinjiang. 【Conclusion】 The main representative varieties are Kenfeng14, Suinong 29, Heinong 62, Heinong 52, Heinong 61. The growth period of Zhonghuang 30, Xinfeng 1, Zhonghuang 54, Heinong 51, Heinong 55 and Heinong 63 is generally around 140 d, which is late-maturing type, and should not be planted in Xinjiang.

Horticultural Special Local Products·Storage and Preservation Processing

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SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars ZHANG Yanfei, Subina Xaokelaiti, YANG Lei, HAO Qing, JIN Juan, FAN Dingyu 2023, 60(7): 1671-1678.  DOI: 10.6048/j.issn.1001-4330.2023.07.013 Abstract ( )   HTML ( )   PDF (729KB) ( )   【Objective】 This experiment aims to construct the fingerprint map on 26 fresh jujube germplasm resources and analyze their genetic diversity in the hope of providing a reference for fresh jujube variety identification and genetic diversity analysis.【Methods】 A total of 26 fresh jujube varieties were analyzed using 22 SSR primers and polymorphic by fluorescent M13 capillary electrophoresis that amplified 130 allelic fragments with an average of 5.909.【Results】 The polymorphic information content (PIC) varied from 0.237,7 to 0.855,6, with an average of 0.59.The heterozygosity for 22 SSR primers ranged from 0.500 to 1.000, with an average of 0.927.The combination of three primer pairs, BFU0308, BFU1157 and BFU0574, could completely distinguish the 26 jujube varieties.【Conclusion】 The genetic similarity coefficient of the 26 varieties is between 0.65 and 1.00, and the 26 varieties can be divided into two major groups at 0.65.

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Effects of extreme high temperature environment on photosynthetic characteristics of Junzao and Fucuimi YANG Xiaojuan, JIN Juan, FAN Dingyu, HAO Qing, YANG Lei, GENG Wenjuan 2023, 60(7): 1679-1688.  DOI: 10.6048/j.issn.1001-4330.2023.07.014 Abstract ( )   HTML ( )   PDF (1604KB) ( )   【Objective】 To study the photosynthetic characteristics of jujube leaves of different varieties in response to extreme high temperature in the hope of laying a foundation for stress resistance, high yield and efficient cultivation of jujube trees.【Methods】 The two varieties with obvious high temperature resistance and the changes of high temperature stress were measured by Li-6400 photosynthetic meter at the day temperature / night temperature of 42℃ / 30℃. 【Results】 High temperature made the stomata of Junzao leaves closed, reduced the gas exchange inside and outside the cells, thus less CO2 going into the cells, and the water utilization efficiency was reduced, both of which could not meet the needs of photosynthesis, resulting in the reduction of leaf photosynthetic rate; The net photosynthetic rate (Pn) of jujube leaves did not change significantly during the continuous 7 d high temperature stress, the net photosynthetic rate and transpiration rate was significantly positively correlated (P<0.01), net photosynthetic rate and intercellular CO2 concentration was of significant negative correlation (P<0.01), so that the stoma was not completely closed, and there was still gas exchange.The leaves transpirated through the pores, taking away part of the heat to reduce the leaf temperature, reducing the impact of high temperature on the photosynthesis of Fucuimi jujube leaves.During the continuous high temperature stress period, the photosynthesis weekly change of the leaves in different branches of the jujube was always higher than that of the Junzao, and the jujube had a stronger ability to adapt to the high temperature stress.The shoots of jujube are more photosynthesis than the leaves of biennial branches, with the strongest ability to adapt to high temperature stress.【Conclusion】 High temperature makes the water utilization efficiency and stomatal conductivity of jujube leaves decrease, which leads to the decrease of photosynthetic rate.The different branches of the leaves have stronger high temperature resistance, among which, the new leaves of Fucuimi have the strongest high temperature resistance, and can best adapt to the high temperature environment.

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Effects of continuous illumination with LED on storage characteristics and sugar metabolism of muskmelon YAO Jun, ZHENG Heyun, ZHANG Cuihuan, Zaituna Maimaiti, GENG Xinli 2023, 60(7): 1689-1697.  DOI: 10.6048/j.issn.1001-4330.2023.07.015 Abstract ( )   HTML ( )   PDF (1346KB) ( )   【Objective】 In order to study the effect of LED monochromatic light on the storage characteristics and sugar content of muskmelon during storage and to develop a new technology for postharvest preservation of muskmelon.【Methods】 The melon variety Xizhoumi25 was used as material, the effects of low light intensity of red, blue and white light-emitting diode on storage characteristics and sugar content of postharvest muskmelon were studied at 7 ℃ cryostat. 【Results】 LED of white light had the best effect on reducing rot and delay the decrease of hardness, and red light had the best effect on keeping the color of melon peel;it was found that the degradation rate of sucrose, glucose and sucrose in muskmelon flesh was delayed in the middle and late stage of storage by LED light treatment, according to the analysis of fructose, fructose and glucose in flesh by High-performance liquid chromatography. White light had the best effect on the sugar content of muskmelon during storage. 【Conclusion】 The results showed that LED delayed the decline of soluble solids content, and weight loss rate, kept the color of melon peel, reduced rot and delayed the decrease of pulp hardness.LED continuous illumination treatment has a significant effect on the preservation and sugar quality maintenance of muskmelon during storage.

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Effects of calcium chloride and 1-MCP on postharvest storage quality of Xizhou melon LI Ziqin, LI Wenqi, JIA Wenting, LI Yuhui, LIU Chengjiang 2023, 60(7): 1698-1704.  DOI: 10.6048/j.issn.1001-4330.2023.07.016 Abstract ( )   HTML ( )   PDF (1079KB) ( )   【Objective】 To investigate the effects of calcium chloride and 1-MCP on the storage quality of postharvest Xizhou honeydew melon.【Methods】 Fresh Xizhou honeydew melon was used as test materials, with 1.5% CaCl2 and 1.5% CaCl2 respectively μL/L 1-methylcyclopropene and 1.5% CaCl2, 1 μL/L 1-methylcyclopropene compound treatment, with the untreated Xizhou melon as the control group.Meanwhile, the changes of decay rate, weight loss rate, respiratory intensity, hardness, soluble solids, protopectin, pod enzyme and CAT enzyme during storage were analyzed.【Results】 Both calcium chloride and 1-MCP could inhibit the respiratory intensity of Xizhou melon, and the combined treatment of calcium chloride and 1-MCP also delayed the respiratory jump of fruit. On the 28th day, the fruit hardness of the combined treatment of calcium chloride and 1-MCP was increased by 83.3% and the original pectin content was increased by 7.5 mg/(g·FW) compared with CK group. Compared with CK, the content of soluble solid increased by 20.4%, and the activities of POD enzyme and CAT enzyme decreased by 42.9% and 64.6%, respectively.【Conclusion】 The combined treatment of calcium chloride and 1-MCP can better inhibit the respiratory intensity of fruit and the decomposition of protopectin, delay the softening of Xizhou melon and maintain the fruit storage quality.

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Effects of vacuum precooling combined with nitrogen packaging treatment on sugar metabolism of Xiaobai apricot TIAN Quanming, LU Fan, YIN Beibei, LIU Xueyan, WEI Jia, WU Bin 2023, 60(7): 1705-1712.  DOI: 10.6048/j.issn.1001-4330.2023.07.017 Abstract ( )   HTML ( )   PDF (1364KB) ( )   【Objective】 To study the regulation effect of N2 packaging treatment on sugar components and sugar metabolism-related enzyme activity in Xiaobai apricot fruits. 【Methods】 With Xinjiang characteristic Xiaobai apricot fruit as the test material, the vacuum pre-cooled combined with nitrogen (N2) packaging was used for treatment, after that, they were stored under 4 ℃ conditions for the storage time of 18 d. 【Results】 The results showed that:vacuum precooling combined with nitrogen treatment could increase the content of glucose and fructose in the apricot, by increasing sucrose phosphate synthase (SPS), sucrose decomposing enzyme (SS-I), neutral invertase (NI), acid invertase (AI) activity, thereby reducing the content of sucrose and sorbitol. When stored for 18 days, the glucose content of the apricot in the vacuum precooling combined with N2 treatment group was 93.42 mg/g and the fructose content was 54.73 mg/g, which increased by 13.32% and 11.6% respectively compared with the control group; In addition to that, the activities of SPS, SS-I, NI, and AI increased by 31.42%, 44.06%, 9.58%, and 19.98%, respectively. 【Conclusion】 The vacuum precooling combined with N2 treatment can maintain the sugars in the Xiaobai apricot, thereby better maintaining the taste of the apricot.

Facility Agriculture·Plant Protection·Forestry

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Effects of different soil moisture on growth, yield, quality and water use efficiency of greenhouse eggplant LIANG Zhiguo, WANG Zepeng, JIA Songnan, FAN Fengcui, LIU Shengyao, ZHANG Zhe, DU Fenghuan, QIN Yong 2023, 60(7): 1713-1721.  DOI: 10.6048/j.issn.1001-4330.2023.07.018 Abstract ( )   HTML ( )   PDF (1158KB) ( )   【Objective】 To provide theoretical basis for precision water-saving irrigation and yield increase of Eggplant in Northern China.【Methods】 The eggplant variety Qieza 2 was taken as the research object, based on the field capacity, five treatments were set respectively:W1:soil water content of 50% - 55% FC, W2:soil water content of 60% - 65% FC, W3:soil water content of 70% - 75% FC, W4:soil water content of 80% - 85% FC, W5:soil water content of 90% - 95% FC, and each treatment was repeated three times.After that, the effects of five different soil moisture on growth, yield, quality and water use efficiency of greenhouse eggplant were studied.【Results】 Under different soil moisture conditions, the plant height and stem diameter of protected eggplant increased first and then decreased with the increase of soil moisture; Under the experimental conditions, the relationship between eggplant yield and irrigation was a quadratic function, y(Y) = -3.202,1x2 + 1,278.5x - 26,511(R2 = 0.994,3).The yield of W4 treatment was the highest, which was 101,029.91 kg/hm2; Reducing soil water content was conducive to the increase of soluble solid, soluble sugar, vitamin C and soluble protein, and also led to the increase of nitrate content; The water use efficiency of protected eggplant increased with the decrease of soil water content.The water use efficiency of W4 treatment with the highest yield was 36.22 kg/m3, decreased by 13.14% compared with W1 treatment, but the yield increased by 54.55%.【Conclusion】 Under the experimental conditions, when the soil water content is 80% - 85% FC, the eggplant yield reaches the maximum, which is 101,029.91 kg/hm2, the nitrite content is the lowest, which is 575.40 mg/kg, and the water use efficiency is relatively high, which is 36.22 kg/m3.It is the most suitable facility eggplant soil water management in Northern China.

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Study on the regeneration characteristics of three kinds of wild fruit trees under spring sowing in Xinyuan wild fruit forest CHENG Gong, LI Yue, LIU Liqiang, YOU Luyao, Mansur Nasir, LIAO Kang 2023, 60(7): 1722-1730.  DOI: 10.6048/j.issn.1001-4330.2023.07.019 Abstract ( )   HTML ( )   PDF (1086KB) ( )   【Objective】 To study the seedling emergence and seedling characteristics of three kinds of wild fruit tree seeds planted artificially in the original habitat and explore the seedling regeneration characteristics of wild fruit trees.【Methods】 After storage in cold stratification, wild apple, wild apricot and wild cherry plum seeds were planted on the flat, shady and sunny slopes of Xinyuan wild fruit forest to observe their emergence and seedling characteristics under natural conditions.【Results】 The emergence period of wild apricot and wild cherry plum were 19 to 33 days after sowing, and the emergence of wild apple began 13 days after sowing.The seedling rate of wild apricot was 38%-72%, the preservation rate was 93%-100%, and the sunny slope was the best; the seedling rate of wild cherry plum was 22.1%-41.8%, the preservation rate was 46.3%-95%, and the shady slope was the best; the seedling rate of wild apple was 1.5%-6.8%, the shady slope was the best, the preservation rate was 57.4%-83.3%, and the sunny slope was the best.The annual seedlings of wild apricot and wild cherry plum grew the fastest in the flat land, and the annual seedlings of wild apple grew faster in the flat land.The seedlings of three kinds of wild fruit trees were suitable to grow in the flat land.【Conclusion】 Both wild apricot and wild cherry plum have higher seedling rate and preservation rate after sowing in spring.They are suitable for live propagation in the original habitat, but it is difficult to sow wild apple.The sunny slope is suitable for the survival of wild apricot seedlings, while the shady slope is suitable for the survival of wild cherry plum seedlings.

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Research status and progress of micro-jet irrigation technology under plastic film in greenhouses XIAO Lingan, MA Yan, SONG Bingwei, JIAO Ruibin, XING Jianfei 2023, 60(7): 1731-1740.  DOI: 10.6048/j.issn.1001-4330.2023.07.020 Abstract ( )   HTML ( )   PDF (1191KB) ( )   【Objective】 To review and summarize the research status and progress of micro-jet irrigation technology under plastic film in greenhouses in the past 20 years, analyze the impact of this technology on greenhouse production in the hope of providing theoretical basis and technical support for its application in facility agriculture water-saving irrigation.【Methods】 The advantages and disadvantages of current greenhouse irrigation were summarized by summarizing domestic and foreign research literature and comparative analysis.The application research status and research results of micro-spraying technology under plastic film in greenhouse irrigation were analyzed and summarized.【Results】 At present, China accounted for 35% of the world’s microirrigation area and scholars mainly studied the influence of spray angle and laying length of microspray belt on soil moisture distribution and crop yield.Submembrane microspray technology were successfully used in field land planting and greenhouse irrigation, including corn, tobacco, sugarcane, fruits and vegetables.Compared with conventional irrigation, drip irrigation under mulch had made some progress based on plant physiology and ecology, and its water-saving effect was slightly inferior to drip irrigation.But it was limited in water source shortage areas, but it has stronger anti-clogging ability, lower installation cost, and was limited to semi-arid areas.【Conclusion】 As a new water-saving irrigation method, under plastic film micro spray irrigation has good anti blocking ability and low cost.It has significant advantages in reducing ground evaporation and improving crop water use efficiency.It is one of the irrigation methods that can effectively control and improve soil secondary salinization.Truly, submembrane microspray irrigation technology can promote the green development of facility agriculture

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Transfer of Hippodamia variegate between licorice and cotton fields based on rubidium marker technology XUE Zhengxuan, CAI Zhiping, ZHANG Zhijian, PENG Tianxiang, HUANG Zhiwei, HUANG Enze, WANG Peiling, LU Yanhui 2023, 60(7): 1741-1747.  DOI: 10.6048/j.issn.1001-4330.2023.07.021 Abstract ( )   HTML ( )   PDF (932KB) ( )   【Objective】 To investigate the transfer of H.variegate between liquorice and neighboring cotton fields and enrich the study of rubidium(Rb)marking method in insect migration and dispersal.【Methods】 Rubidium marker technique was used to detect the retention period of rubidium in H.variegate indoors; and rubidium marking method was applied to explore the migration range of H.variegate from liquorice strip to cotton field in the field.【Results】 The retention period of rubidium in H.variegate adult was about 15 d. The migration range of H.variegate adult from liquorice to cotton fields differed, with detection rates of Rb in adults of 0-15 m > 30-50 m > 15-30 m > 50-100 m.【Conclusion】 Rubidium marking method can effectively track the migration of adult and larvae of H.variegate in the field.

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Occurrence of Ampelomyces -hyperparasites of Erysiphales infesting iandscapeplant and optimizing isolation method TANG Xiaoxue, SUN Lin, Hazila Saiyilihan, LI Wenli, DONG Peizhu, REN Yuzhong, LI Guoying 2023, 60(7): 1748-1755.  DOI: 10.6048/j.issn.1001-4330.2023.07.022 Abstract ( )   HTML ( )   PDF (873KB) ( )   【Objective】 Occurrence of Ampelomyces -hyperparasites of Erysiphales infesting iandscapeplant and optimizing isolation method.【Methods】 In 2020, white powdery mildew parasite infesting 18 landscape plant species in Shihezi area were investigated, the isolation method of Ampelomyces quisqualis on the collected samples was optimized, and the medium suitable for isolating Ampelomyces was selected.【Results】 The presence of Ampelomyces was detected on 17.The higher number of the samples with mycoparasites was noted in the case of compositae (the incidence of parasitism by A.quisqualis was 40.92).The highest number of the samples with mycoparasites was noted in the case of Golovinomyces (43.59 of the samples).The isolation rate of the water agar filter pickingpycnidia (90%) method was significantly (P<0.01) higher than that of direct pickingpycnidia with needle (30%) and reduced the contamination.The Ampelomyces were all able to grow on six medium, Czapek, PDA, PSA and their added malt extracts respectively, with isolation rates above 90.0%.But there were significant differences in the growth rate on different media.The growth rate of the colonies was significantly(P<0.05) enhanced due to the addition of malt extract, where the modified PSA and Czapek medium were more favorable for the isolation of Ampelomyces.【Conclusion】 The high participation of the Ampelomyces is noted in the powdery mildew of the study.The water agar filter pickingpycnidia +the modified PSA and Czapek medium adding malt extract is effective method to isolate Ampelomyces.

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Pollination biology of Tamarix karelinii Bunge(Tamaricaceae) Sailitanati Saidakebaier, ZHONG Wen, YU Runze 2023, 60(7): 1756-1765.  DOI: 10.6048/j.issn.1001-4330.2023.07.023 Abstract ( )   HTML ( )   PDF (1605KB) ( )   【Objective】 Pollination biology of Tamarix karelinii Bunge(Tamaricaceae).【Methods】 Tamarix karelinii Bunge was used as the research object, and the morphological characteristics of flowers, flowering dynamics, pollinating insect species, and their pollination behaviors were recorded through field observations, the sugar content of nectar was measured by centrifugation, pollen viability was determined by the TTC method, receptivity of stigma was determined by MTT method, and the pollen carrying capacity of pollinators was counted by microscopic counting.【Results】 The population of Tamarix karelinii Bunge had a flowering period about 58 days and a single flowering period was 3 days.During the flowering period of a single flower, both pollen and stigma were active, and their activity was the highest on day of flowering.The Apis mellifera, Lasioglossum albescens, Eristalis tenax, Eupeodes corollae and the Lucilia sericata were the main pollinators, with the Lasioglossum albescens having the highest flower-visiting frequency, the Apis mellifera an absolute advantage in terms of the number of individuals, single flower stay time and pollen carrying capacity; The sugar content of nectar was the highest during 14:00-16:00 of the day, which coincided with the peak of flower visitation by pollinators as mentioned above.【Conclusion】 T.karelinii has floral syndromes adapted to insect pollination, and the generalized pollination system is suitable for Hymenoptera and Diptera insects to pollinate it, among which the pollination ability of Apis mellifera is more significant.

Microbes·Animal Husbandry Veterinarian

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Isolation, identification and activity of halophilic bacteria from Dabancheng Salt Lake, Xinjiang LI Yixin, CHEN Yong, LIU Xiaolu, Ainijiang Ersiman, XU Lijuan, LIU Qianqian, BAO Xiaowei, SONG Suqin 2023, 60(7): 1766-1772.  DOI: 10.6048/j.issn.1001-4330.2023.07.024 Abstract ( )   HTML ( )   PDF (914KB) ( )   【Objective】 To study the resources of halophilic microorganisms in Salt Lake of Xinjiang and explore the agricultural and medical activities of the fermentation broth of the strain.【Methods】 In this study, the sludge samples from Dabancheng Salt Lake in Daban City were collected for isolation and identification of bacterial strains.Four different separation media were used for isolation and identification by plate dilution coating method combined with 16S rRNA.The antagonistic activity of the isolated actinomycetes was studied by flat-plate antagonistic method, and the anticancer activity of the isolated actinomycetes was determined by HepG2 cell proliferation.【Results】 Eighteen strains of bacteria were obtained and identified as 14 genera, among which Bacillus, Streptomyces and Nocardiopsis were the dominant species.Three actinomycetes showed antagonistic activity against plant pathogens, among which strains Tsukamurella sinensis DBC26 and Nocardiopsis crassaminis DBC31 showed antagonistic activity against fusarium oxysporum. Streptomyces longispororuber DBC5 showed antagonistic activity against rice vaccinia.The anti-tumor activity of DBC5, DBC9 and DBC10 strains against HepG2 cell proliferation was 96.4%, 91.8% and 87.8%, respectively.【Conclusion】 This study showed that the number and species of bacteria in Dabancheng Salt Lake are rich in diversity, and rare actinomycetes are rich in species, and their fermentation broth has the activity of antagonizing plant pathogens and inhibiting tumor cell proliferation.

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Study on the solid-state fermentation technology of yeast 0939-5 LI Huajing, WANG Xiaodong 2023, 60(7): 1773-1779.  DOI: 10.6048/j.issn.1001-4330.2023.07.025 Abstract ( )   HTML ( )   PDF (1139KB) ( )   【Objective】 To determine the optimal solid-state fermentation substrate and culture conditions for the production of volatile antibacterial substances by using the yeast strain 0939-5 as the test material.【Methods】 The solid nutrients and culture conditions of the strain were optimized by single factor method.【Results】 The optimum solid-state fermentation substrate of yeast strain 0939-5 was corn meal, the optimum carbon source concentration was glucose 2%, and the optimum inorganic salt concentration was 0.1% MgSO4.The optimum conditions for solid-state fermentation of yeast strain 0939-5 were inoculation concentration 1×108 CFU/mL, inoculation volume 10%, initial pH 4, and initial water content 40%.【Conclusion】 On the basis of the basal medium, corn meal was used as a solid fermentation substrate, and 2% glucose and 0.1% MgSO4 were added respectively, and the optimumculture conditions were inoculum concentration of 1×108 CFU/mL, the inoculum volume of 10%, the initial pH of 4, and the initial water content 40% at 25℃ for 5 days.

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Changes of related proteins and phenylpropane metabolic enzymes in melon infected by Alternaria alternata LIU Yang, BAI Yujia, ZHANG Zhengxiao, CHU Chenli, WANG Tiantian, FENG Zuoshan 2023, 60(7): 1780-1789.  DOI: 10.6048/j.issn.1001-4330.2023.07.026 Abstract ( )   HTML ( )   PDF (2082KB) ( )   【Objective】 To analyze the differences of related proteins and enzymes involved in phenylpropanoid metabolism in pathogenesis from diverse melons’ peel and pulp tissues, which are stored in room temperature (25℃) and explore the endogenous disease resistance of melons stored at room temperature.【Methods】 Melon was were inoculated with Alternaria alternata, and their epricarp, endocarp and pulp tissues were separated during the storage. The pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in different tissues of muskmelons were measured in different storage days,and the disease resistance of different tissues of melon were analyzed. The indicators in these issues were measured every other day to deduce their the disease resistance. Compared with previous researches, the effects of different storage temperatures on disease resistance of muskmelon were figured out as well.【Results】 During storage, the lesion diameter of Jiashigua was smaller than 86-1 melon’s, the lesion size in the peel and pulp of 86-1 melons reached 1.55 and 1.375 times bigger than Jiashigua’s on the 16th day. Activities of Chitinase (CHT) and β-1,3-glucanase (GLU) in melon showed a trend of increasing first and then decreasing, and the enzyme activity of Jiashigua was higher than 86-1 melon’s, exocarp was higher than endocarp and pulp tissue. Compared to 86-1 melon, Jiashigua reached the peak of enzyme activity in a shorter time.【Conclusion】 It is found that temperature has no obvious effect on the change trend of the pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in melons, and enzyme activities all show a trend of increasing at first and then decreasing; In terms of disease resistance, Jiashigua is superior to 86-1 melon. In dealing with the infection of Alternaria alternata, peels have stronger disease resistance than pulps do, so Jiashigua is more active to resist than 86-1 melon facing the infection of Alternaria alternata.

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Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix CHEN Yihuang, XING Li, DONG Zhenzhen, MA Xiaomei, HUANG Jianjun, LUO Xiaoxia 2023, 60(7): 1790-1797.  DOI: 10.6048/j.issn.1001-4330.2023.07.027 Abstract ( )   HTML ( )   PDF (1006KB) ( )   【Objective】 To lay a foundation for the prevention and control of major agricultural and animal husbandry diseases in Xinjiang.【Methods】 Ten species of actinomycetes from rhizosphere soils of Tamarix ramosissima in southern Xinjiang were selected and their antibactieral activities were studied.Species diversity of actinomycetes in rhizosphere soil of Tamarix ramosissima was analyzed by 16SrDNA sequencing using four kinds of media.The antibacterial activity was screened by plate confrontation methods.The antibacterial activity was screened by plate confrontation method.【Results】 The results showed that total 294 strains were isolated, and total 150 strains were identified, which belonged to seven classes and twelve orders, total 12 families, total 24 genera, and 9 potential new species.At the same time, the antibacterial activity was analyzed with Staphylococcus aureus, Pseudonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Erwinia amylohydrolytic, Slmonella typhoid, Candida albicans, Chain bacteria Verticilliuma dhiiae, Fusarium oxysporum as the target bacteria.【Conclusion】 Total 68 strains of active actinmycetes were screened by antibacterial activity analysis. Isolation of actinomycetes from rhizosphere soil of Tamarix ramosissima enriched microbial resources and species reserve.

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Ecological characteristics of Diptychus maculates in Turks River YAO Na, GE Jianmin, WANG Chengxin, SONG Yong, CHEN Shengao, YANG Haoke, XIE Congxin, WEI Qi 2023, 60(7): 1798-1805.  DOI: 10.6048/j.issn.1001-4330.2023.07.028 Abstract ( )   HTML ( )   PDF (1261KB) ( )   【Objective】 To explore the morphological characteristics, age, growth and other ecological characteristics of spotted Diptychus maculates in Turks River.【Methods】 From 2019 to 2021, through the sampling and observation of the Turks river spotted Diptychus maculates., classical biological measurement methods were used to identify the age and analyze its growth characteristics.【Results】 The body of Turks Rriver spotted double-lipped fish was elongated, with fine scales, and the lateral line was complete.The morphological characteristics were different from other water systems.The color of the back was gray-brown, with different black spots, and the abdomen was light yellow and grayish-white.The average body length was (143.66±57.94) mm, the average weight was (64.81±92.10) g, and the average age (4.08±2.49*); The fitting relationship between body length and weight was W =5.80×10-6L3.165,3(R2=0.952,1), which belonged to allometric growth; Body length was highly correlated with body height, head length, snout length, eye distance, caudal stalk length, and caudal stalk height, but had a lower correlation with body width and.The progressive body length (n=120) was L∞=346.79 mm, k=0.09, W∞=634.97 g, t 0 = - 0.34, ti=12.46.Growth equation of length and weight: Lt = 346.79 (1-e-0.09 (t + 0.34)),Wt = 634.97 (1-e-0.09 (t + 0.34))3.165,3.【Conclusion】 The morphology of Turks river spotted Diptychus maculates is different from other water systems.It is a plateau cold-water fish with allometric growth, poor adaptability, old inflection point, slow growth, and unstable growth characteristics.

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Analysis of sequence analysis of pD353 plasmids of Staphylococcus aureus D353, the source of cattle and sheep in Xinjiang HE Tengfei, LIU Yingyu, ZHANG Liuqing, CHEN Wang, LI Zeya, HU Yun, JIANG Jindou, Zulihumaer Aili 2023, 60(7): 1806-1812.  DOI: 10.6048/j.issn.1001-4330.2023.07.029 Abstract ( )   HTML ( )   PDF (1021KB) ( )   【Objective】 To analyze the wild plasmid sequence characteristics of Staphylococcus aureus methicillin-resistant strains and vancomycin-resistant strains in the hope of helping the study of the formation of super-drug-resistant bacteria.【Methods】 The multidrug-resistant strain D353 was identified by 16s rRNA, and the sequence features, genes, function and replication of the plasmid pD353 were analyzed by BLAST, MEGA 11, Mauve, and trf.【Results】 The results showed that the plasmid size from pD353 was 34,063 bp, the content of G-C mol was 30.42%.Plasmid sequence analysis found that pD353 plasmid and Staphylococcus aureus plasmid sequence pIT1-S and pIT4-R had a high homologous, which was rich in heavy metal transport protein and insertion sequence translocation enzyme, its rep protein and pSK639 family plasmids had a high homologous.【Conclusion】 Series repeat sequence analysis initially concluds that the plasmid is a θ replication and antibiotic resistance gene is unfound.

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Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region FENG Qian, HE Qi, LI Xue, YI Yuanyang, SUN Jian, ZHU Jing, GU Meiying, TAN Huilin, ZHANG Zhidong 2023, 60(7): 1813-1820.  DOI: 10.6048/j.issn.1001-4330.2023.07.030 Abstract ( )   HTML ( )   PDF (1197KB) ( )   【Objective】 To optimize the extraction conditions of polysaccharides from the wild boletus in Aksu region and determine the physical properties and antioxidant capacity of different polysaccharide components. 【Methods】 The wild boletus was sampled from Aksu region,and water extraction conditions of polysaccharide were optimized. Two polysaccharide components,LD-40 and LD-80,were obtain by fractional precipitation of ethanol with volume fraction of 40% and 80%. The polysaccharide properties of water solubility,water retention and viscosity were tested. The antioxidant capacity was evaluated by free radical scavenging of DPPH and hydroxyl and reducing power experiments. 【Results】 The optimal extraction conditions were solid-liquid ratio 1∶30,temperature at 80℃,time for 3 h,then the yield of polysaccharide was 13.36%. Polysaccharide components,LD-40 and LD-80,obtained from ethanol fractional precipitation accounted for 92.37% and 7.63% of the total polysaccharide,respectively. The water solubility of LD-40 and LD-80 was 66.9% and 74.9%,respectively,and the water holding capacities were 7.71 and 6.16 g/g,respectively. At the same concentration,the viscosity of LD-40 was stronger than that of LD-80,but LD-80 had stronger antioxidant capacity than LD-40.【Conclusion】 The wild boletus in Aksu region is rich in polysaccharides which has good water holding capacity,but the antioxidant activity is weak. The results can provide scientific reference for the further development and utilization of wild boletus polysaccharides.