Abstract: 【Objective】 To study the resources of halophilic microorganisms in Salt Lake of Xinjiang and explore the agricultural and medical activities of the fermentation broth of the strain. 【Methods】 In this study, the sludge samples from Dabancheng Salt Lake in Daban City were collected for isolation and identification of bacterial strains.Four different separation media were used for isolation and identification by plate dilution coating method combined with 16S rRNA.The antagonistic activity of the isolated actinomycetes was studied by flat-plate antagonistic method, and the anticancer activity of the isolated actinomycetes was determined by HepG2 cell proliferation. 【Results】 Eighteen strains of bacteria were obtained and identified as 14 genera, among which Bacillus, Streptomyces and Nocardiopsis were the dominant species.Three actinomycetes showed antagonistic activity against plant pathogens, among which strains Tsukamurella sinensis DBC26 and Nocardiopsis crassaminis DBC31 showed antagonistic activity against fusarium oxysporum. Streptomyces longispororuber DBC5 showed antagonistic activity against rice vaccinia.The anti-tumor activity of DBC5, DBC9 and DBC10 strains against HepG2 cell proliferation was 96.4%, 91.8% and 87.8%, respectively. 【Conclusion】 This study showed that the number and species of bacteria in Dabancheng Salt Lake are rich in diversity, and rare actinomycetes are rich in species, and their fermentation broth has the activity of antagonizing plant pathogens and inhibiting tumor cell proliferation.