Abstract: 【Objective】 In order to explore the effects of biological pesticide combinations on cotton Verticillium wilt control and growth, and provide theoretical basis for the healthy growth of cotton plants. 【Methods】 A field experiment was conducted in 2022, with Xinluzhong 42 as the experimental variety and drip application as the main method applying 80% ethyl allicin emulsifiable concentrate of 1.5 kg/hm² and potassium phosphite of 15 L/hm² based on a glutathione content of 0.3 kg/hm², they were compounded with antiviral agents (T₁, 0.15 L/hm²), composite microbial fertilizer (T₂, 30 L/hm²), 15% oxamycin (T₃, 2.25 kg/hm²), 5% octambumide acetate (T₄, 1.5 kg/hm²), and 25% pyrazolin (T₅, 2.25 L/hm²), respectively; The effects of different pesticides on cotton disease control, agronomic traits, and yield were analyzed. 【Results】 The results showed that each treatment had significant control effects on cotton diseases, promoted cotton growth to a certain extent, and increased cotton plant height, number of fruit branches, and number of buds and bolls. The control effect of T₂ treatment on cotton diseases was 19.99% and 23.34% higher than that of T₁ and T₃ treatment, and the number of bolls in T₁ and T₂ treatment increased by 1.8 and 2.67 compared to CK treatment. The seed cotton yield of T₂ treatment was 6,104.86 kg/hm², which had the highest yield and reached significance than other treatments. 【Conclusion】 Under the experimental conditions, drip application of compound microbial fertilizer+80% acetoallin EC+potassium phosphite+glutathione (T₂) can improve the disease control effect to a certain extent, increase the number of buds and bolls per plant, and significantly increase yield.